TetR 蛋白, E.coli (His)

TetR protein acts as a repressor of the tetracycline resistance element and utilizes the helix-turn-helix structure of its N-terminal region for DNA binding. Tetracycline binding induces a conformational change in TetR that reduces its affinity to the tetA promoter operator site. TetR Protein, E.coli (His) is the recombinant E.coli-derived TetR, expressed by E. coli , with N-6*His labeled tag. ,

TetR 蛋白作为四环素抗性元件的阻遏物，其 N 末端区域采用螺旋-转角-螺旋结构，有利于 DNA 结合。四环素与 TetR 结合会引起构象变化，从而降低阻遏蛋白对四环素抗性基因 (tetA) 启动子操纵位点的亲和力。TetR 和四环素之间的这种动态相互作用作为一种调节机制，调节与四环素耐药性相关的基因的表达。在四环素存在的情况下，TetR 的结合特性会发生改变，从而消除 tetA 启动子的抑制，并使参与四环素抗性的基因得以表达。

E.coli

E. coli

N-6*His

P04483 (M1-S207)

56882117

MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS

Approximately 24.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCL, 500 mM NaCl, pH 8.0, 8% trehalose, 8% mannitol and 0.02% Tween80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.