1. 重组蛋白
  2. CD Antigens Biotinylated Proteins
  3. Endothelial cell CD Proteins
  4. TFR-1/CD71
  5. TFRC 蛋白, Mouse (Biotinylated, HEK293, His)

TFRC 蛋白, Mouse (Biotinylated, HEK293, His)

目录号: HY-P701303
COA 产品使用指南

TFRC 是细胞铁吸收的关键蛋白,协调受体介导的配体转铁蛋白的内吞作用进入专门的内体,内体酸化触发铁释放。TFRC 通过铁摄取积极调节 T 细胞和 B 细胞增殖。TFRC 蛋白, Mouse (Biotinylated, HEK293, His) 是重组的 TFRC 蛋白,由 HEK293 表达,带有 His 标签。

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SPR 检测服务   重组蛋白定制服务

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     可免费申领三个不同产品的试用装。

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描述

TFRC is a key protein in cellular iron uptake, coordinating receptor-mediated endocytosis of the ligand transferrin into specialized endosomes, where endosomal acidification triggers iron release.TFRC actively regulates T and B cell proliferation through iron uptake.TFRC Protein, Mouse (Biotinylated, HEK293, His) is the recombinant mouse-derived TFRC protein, expressed by HEK293 , with His labeled tag.

研究背景

TFRC 是细胞铁吸收的关键蛋白,协调受体介导的配体转铁蛋白的内吞作用进入专门的内体,内体酸化触发铁释放。脱铁转铁蛋白-受体复合物在pH值恢复到中性的同时循环到细胞表面,导致脱铁转铁蛋白对其受体的亲和力丧失。TFRC 对红细胞和神经系统发育至关重要,通过铁的摄取积极调节 T 细胞和 B 细胞增殖。作为脂质传感器,TFRC 通过调节 JNK 通路来调节线粒体融合。低膳食硬脂酸水平会诱导 JNK 通路激活,导致 HUWE1 介导的泛素化和线粒体融合蛋白 MFN2 的降解,从而抑制线粒体融合。相反,高硬脂酸水平会使 TFRC 硬脂酰化,抑制 JNK 通路激活并防止 MFN2 降解。TFRC 还可以作为具有二硫键的同型二聚体,每个亚基结合一个转铁蛋白分子,并与 SH3BP4 和 STEAP3 相互作用,后者促进红系前体细胞中的 TFRC 内吞作用。

种属

Mouse

表达系统

HEK293

标签

His

蛋白编号

Q62351 (C89-F763)

基因 ID

22042

同用名
CD71; TFRC; TFR; sTfR; TfR1; Trfr; T9; p90
分子量

80-100 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

TFRC Protein, Mouse (Biotinylated, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
TFRC Protein, Mouse (Biotinylated, HEK293, His)
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HY-P701303
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