1. 重组蛋白
  2. Cytokines and Growth Factors
  3. TGF-beta Superfamily Neurotrophic Factors
  4. TGF-β TGF-β
  5. TGF-β1
  6. TGF beta 1/TGFB1 蛋白, Rhesus Macaque (HEK293, His)

TGF beta 1/TGFB1 蛋白, Rhesus Macaque (HEK293, His)

目录号: HY-P77856
产品使用指南

转化生长因子 β-1 (TGF-β-1) 前蛋白包含潜伏相关肽 (LAP) 和活性 TGF-β-1 链,形成具有二硫键的同二聚体。激活后,蛋白水解过程会释放成熟的 TGF-β-1,使其在细胞生长、分化和免疫反应中发挥调节功能。二聚体结构强调了 LAP 和 TGF-β-1 在生物系统内协调多方面活动时复杂的相互作用。TGF beta 1/TGFB1 蛋白, Rhesus Macaque (HEK293, His) 是重组的 TGF beta 1/TGFB1 蛋白,由 HEK293 表达,带有 N-His 标签和 C33S 突变。TGF beta 1/TGFB1 蛋白, Rhesus Macaque (HEK293, His) 全长 361 个氨基酸,分子量为 40-50/14-15 kDa。

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描述

Transforming growth factor beta-1 (TGF-beta-1) proprotein, comprising Latency-associated peptide (LAP) and active TGF-beta-1 chains, forms a homodimer with disulfide linkages. Upon activation, proteolytic processing releases mature TGF-beta-1, enabling its regulatory functions in cell growth, differentiation, and immune responses. The dimeric structure emphasizes LAP and TGF-beta-1's intricate interplay in orchestrating multifaceted activities within biological systems. TGF beta 1/TGFB1 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived TGF beta 1/TGFB1 protein, expressed by HEK293 , with N-His labeled tag and C33S mutation. The total length of TGF beta 1/TGFB1 Protein, Rhesus Macaque (HEK293, His) is 361 a.a., with molecular weight of 40-50/14-15 kDa.

研究背景

转化生长因子 β-1 (TGF-β-1) 前蛋白是潜伏相关肽 (LAP) 和转化生长因子 β-1 (TGF-β-1) 链的前体,其中 LAP 充当调节亚基TGF-β-1作为活性亚基。前蛋白形成具有二硫键的同型二聚体,激活后会经历蛋白水解过程,释放成熟的 TGF-β-1,使其在各种细胞过程中发挥调节功能,包括细胞生长、分化和免疫反应。二聚体结构强调了 LAP 和 TGF-β-1 之间复杂的相互作用在生物系统内协调 TGF-β-1 的多方面活动中的重要性。

种属

Rhesus Macaque

表达系统

HEK293

标签

N-His

蛋白编号

F7HCV5 (L30-S390, C33S)

基因 ID
蛋白结构
N-term
His
TGFB1 (L30-S390, C33S)
Accession # F7HCV5
C-term
同用名
CEDLAP; DPD1; TGF beta1; TGFB; TGFB1; TGFbeta; TGF-beta-1
分子量

40-50/14-15 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
TGF beta 1/TGFB1 Protein, Rhesus Macaque (HEK293, His)
目录号:
HY-P77856
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