TGF beta 3/TGFB3 蛋白, Human (HEK293, Avi)

Latent transforming growth factor beta-3 (TGF-beta-3) preprotein serves as a precursor to latency-associated peptide (LAP) and active TGF-beta-3 chains, which serve as regulatory and functional subunits. It plays a crucial role in maintaining the latent state of TGF-β-3 within the extracellular matrix. TGF beta 3/TGFB3 Protein, Human (HEK293, Avi) is the recombinant human-derived TGF beta 3/TGFB3 protein, expressed by HEK293 , with C-Avi labeled tag. The total length of TGF beta 3/TGFB3 Protein, Human (HEK293, Avi) is 112 a.a., with molecular weight of 15-20 kDa.

潜在转化生长因子 β-3 (TGF-β-3) 前蛋白是潜伏相关肽 (LAP) 和活性 TGF-β-3 链的前体，分别充当调节亚基和功能亚基。它在维持细胞外基质内 TGF-β-3 的潜伏状态方面发挥着至关重要的作用。通过与 TGF-β-3 的非共价结合，潜在 TGF-β-3 通过与 LTBP1 和 LRRC32/GARP 等关键“环境分子”相互作用，主动调节激活过程。这些相互作用有助于 TGF-β-3 的受控激活，其中 LTBP1 和 LRRC32/GARP 是该调节机制中的关键组成部分。此外，与整合素的相互作用会诱导潜伏期相关肽链的结构变化，导致随后释放活性 TGF-β-3。这种复杂的分子相互作用强调了潜在 TGF-β-3 在各种生理环境中协调 TGF-β-3 的调节激活中的关键作用。

Immobilized Human TGF-beta RII, mFc Tag at 0.5 μg/mL (100μl/well) on the plate. Dose response curve for Biotinylated Human Mature TGF beta 3, Avi Tag with the EC₅₀ of ≤15 ng/mL determined by ELISA.

Human

HEK293

C-Avi

P10600-1 (A301-S412)

/

15-20 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of 4 mM HCl. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in 4 mM HCl, pH 3.0.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.