THBS1 蛋白, Human (HEK293, His)

The THBS1 protein is an adhesion glycoprotein that controls multiple cellular processes and mediates cell-cell and cell-matrix interactions. It plays multifunctional roles in inflammation, angiogenesis, wound healing, ROS and NO signaling, apoptosis, senescence, senescence, self-renewal, stemness, and cardiovascular/metabolic homeostasis. THBS1 Protein, Human (HEK293, His) is the recombinant human-derived THBS1 protein, expressed by HEK293 , with C-10*His labeled tag.

THBS1 蛋白是一种粘附糖蛋白，通过介导细胞与细胞以及细胞与基质的相互作用来协调无数的细胞过程。它具有多功能作用，复杂地参与炎症、血管生成、伤口愈合、活性氧 (ROS) 和一氧化二氮 (NO) 信号传导、细胞凋亡、衰老、衰老、细胞自我更新、干性以及心血管和心血管系统的维护。代谢稳态。这种多功能蛋白质负向调节树突状细胞活化和细胞因子释放，有助于炎症消退和免疫稳态。THBS1 作为受体 CD47 和 CD36 的配体，在调节一氧化二氮 (NO) 信号传导和血压调节中发挥着关键作用。此外，它还通过激活 NADPH 氧化酶 NOX1 影响内皮细胞衰老，并导致过量 ROS 产生。THBS1 进一步抑制干细胞自我更新，调节伤口愈合，诱导足细胞凋亡，并通过与 CD47 和 CD36 的相互作用抑制血管生成。该蛋白质参与各种细胞过程，强调了其在生理和病理条件下的重要性，从肥胖的代谢功能障碍到内质网应激反应。作为具有二硫键的同源三聚体，THBS1 对各种细胞外基质成分和整合素表现出结合亲和力，强调了其在细胞相互作用和信号传导途径中的关键作用。

Measured by its binding ability in a functional ELISA. When Recombinant Human VEGF183 Protein is immobilized at 1 µg/mL (100µL/well) can bind Human THBS1 Protein. The ED₅₀ for this effect is 382.6 ng/mL.

Human

HEK293

C-10*His

P07996 (N19-P1170)

7057  [NCBI]

NRIPESGGDNSVFDIFELTGAARKGSGRRLVKGPDPSSPAFRIEDANLIPPVPDDKFQDLVDAVRAEKGFLLLASLRQMKKTRGTLLALERKDHSGQVFSVVSNGKAGTLDLSLTVQGKQHVVSVEEALLATGQWKSITLFVQEDRAQLYIDCEKMENAELDVPIQSVFTRDLASIARLRIAKGGVNDNFQGVLQNVRFVFGTTPEDILRNKGCSSSTSVLLTLDNNVVNGSSPAIRTNYIGHKTKDLQAICGISCDELSSMVLELRGLRTIVTTLQDSIRKVTEENKELANELRRPPLCYHNGVQYRNNEEWTVDSCTECHCQNSVTICKKVSCPIMPCSNATVPDGECCPRCWPSDSADDGWSPWSEWTSCSTSCGNGIQQRGRSCDSLNNRCEGSSVQTRTCHIQECDKRFKQDGGWSHWSPWSSCSVTCGDGVITRIRLCNSPSPQMNGKPCEGEARETKACKKDACPINGGWGPWSPWDICSVTCGGGVQKRSRLCNNPTPQFGGKDCVGDVTENQICNKQDCPIDGCLSNPCFAGVKCTSYPDGSWKCGACPPGYSGNGIQCTDVDECKEVPDACFNHNGEHRCENTDPGYNCLPCPPRFTGSQPFGQGVEHATANKQVCKPRNPCTDGTHDCNKNAKCNYLGHYSDPMYRCECKPGYAGNGIICGEDTDLDGWPNENLVCVANATYHCKKDNCPNLPNSGQEDYDKDGIGDACDDDDDNDKIPDDRDNCPFHYNPAQYDYDRDDVGDRCDNCPYNHNPDQADTDNNGEGDACAADIDGDGILNERDNCQYVYNVDQRDTDMDGVGDQCDNCPLEHNPDQLDSDSDRIGDTCDNNQDIDEDGHQNNLDNCPYVPNANQADHDKDGKGDACDHDDDNDGIPDDKDNCRLVPNPDQKDSDGDGRGDACKDDFDHDSVPDIDDICPENVDISETDFRRFQMIPLDPKGTSQNDPNWVVRHQGKELVQTVNCDPGLAVGYDEFNAVDFSGTFFINTERDDDYAGFVFGYQSSSRFYVVMWKQVTQSYWDTNPTRAQGYSGLSVKVVNSTTGPGEHLRNALWHTGNTPGQVRTLWHDPRHIGWKDFTAYRWRLSHRPKTGFIRVVMYEGKKIMADSGPIYDKTYAGGRLGLFVFSQEMVFFSDLKYECRDP

Approximately 130 kDa

Greater than 83% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 8% Trehalose, 4% Mannitol, 200 mM NaCl, 0.02% Tween80, pH 6.5 or PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.