Thioredoxin/TXN 蛋白, Human (Solution)

Thioredoxin/TXN protein participates in multiple redox reactions, utilizing its active center dithiol for reversible oxidation and disulfide bond formation. It catalyzes important dithiol-disulfide exchange and plays a key role in S-nitrosylation of cysteine residues in response to intracellular nitric oxide. Thioredoxin/TXN Protein, Human (Solution) is the recombinant human-derived Thioredoxin/TXN protein, expressed by E. coli , with tag free. The total length of Thioredoxin/TXN Protein, Human (Solution) is 105 a.a., with molecular weight of ~14 kDa.

硫氧还蛋白/TXN 蛋白积极参与多种氧化还原反应，利用其活性中心二硫醇的可逆氧化形成二硫键，并催化关键的二硫醇-二硫键交换反应。除了其经典的氧化还原功能外，硫氧还蛋白在靶蛋白内半胱氨酸残基的可逆 S-亚硝基化中发挥着关键作用，有助于细胞对细胞内一氧化氮的反应。值得注意的是，它通过响应一氧化氮对 CASP3 活性位点半胱氨酸进行亚硝基化来对 caspase-3 活性进行调节控制。此外，硫氧还蛋白显示其对电离辐射细胞中 FOS/JUN AP-1 DNA 结合活性的影响，通过其氧化/还原状态调节 AP-1 转录活性。此外，硫氧还蛋白还参与白细胞介素 2 受体 TAC (IL2R/P55) 表达的增强，突出了其在氧化还原调节之外的细胞过程中的多方面作用。

Measured by its ability to catalyze the reduction of insulin. The reaction leads toprecipitation, which can be measured by absorbance at 650 nm and the specific activity is 5-10 A650/min/mg.

Human

E. coli

Tag Free

P10599 (M1-V105)

7295  [NCBI]

Approximately 14 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.2 μm filtered solution of PBS, pH 7.5

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.