Thioredoxin/TXN 蛋白, E.coli (His)

Thioredoxin/TXN Protein, E.coli (His) is a hydrogen carrier protein and exists widely in organism. Thioredoxin suppression disbalances insulin responsiveness in chicken cardiomyocytes through PI3K/Akt pathway inhibition^[1][2].

硫氧还蛋白（Txn）是一种氢载体蛋白，广泛存在于生物体内。已证实 Txn 缺乏会导致心肌细胞损伤。硫氧还蛋白（Txn）系统是细胞内最重要的抗氧化防御机制，由Txn、硫氧还蛋白还原酶（TR）和烟酰胺腺嘌呤二核苷酸磷酸（NADPH）组成。Txn 系统可以氧化还原调节心脏中胰岛素依赖性葡萄糖代谢，对细胞活力至关重要^[1][2]。

Its ability to catalyze the reduction of insuline by precipitation reaction measured by absorbance at 650 nm. The specific activity is 3 units/mg, as measured under the described conditions.

E.coli

E. coli

C-His

M26133 (M1-A109)

948289  [NCBI]

MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLDANLA

Approximately 13 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filter solution of 50 mM Tris-HCl, 0.15 M NaCl, 1 mM EDTA, pH 7.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Yang J, et, al. Dysfunction of thioredoxin triggers inflammation through activation of autophagy in chicken cardiomyocytes. Biofactors. 2020 Jul;46(4):579-590.  [Content Brief]

  [2]. Yang J, et, al. Selenium deficiency-induced thioredoxin suppression and thioredoxin knock down disbalanced insulin responsiveness in chicken cardiomyocytes through PI3K/Akt pathway inhibition. Cell Signal. 2017 Oct;38:192-200.  [Content Brief]