1. 重组蛋白
  2. Cytokines and Growth Factors CD Antigens Receptor Proteins Enzymes & Regulators
  3. Angiopoietins Endothelial cell CD Proteins Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. TIE-2 TIE Receptors
  5. TIE-2
  6. TIE-2 蛋白, Mouse (HEK293, His)

TIE-2 蛋白, Mouse (HEK293, His)

目录号: HY-P73610
COA 产品使用指南

TIE-2 蛋白, Mouse (HEK293, His) 属于受体酪氨酸激酶家族,作为血管生成素-1 (Ang1) 的受体发挥作用。Ang1 在细胞与细胞接触处桥接 TIE-2,导致在细胞与细胞接触存在的情况下 TIE-2 发生反式关联。TIE-2 激活通过介导 EC 的生存信号和调节周细胞的募集来促进血管组装和成熟。TIE-2通过增强内皮屏障功能和抑制内皮细胞凋亡来维持成熟血管的血管完整性。TIE-2 蛋白, Mouse (HEK293, His) 是重组的 TIE-2 蛋白,由 HEK293 表达,带有 C-His 标签。TIE-2 蛋白, Mouse (HEK293, His) 全长 722 个氨基酸,分子量为 95-120 kDa。

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规格 价格 是否有货 数量
Free Sample   Apply now  
2 μg ¥350 In-stock
10 μg ¥750 In-stock
50 μg ¥1650 In-stock
100 μg ¥2600 In-stock
500 μg ¥8500 In-stock
1 mg ¥15000 询价
> 1 mg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

TIE-2 Protein, Mouse (HEK293, His) belongs to the receptor tyrosine kinase family and functions as a receptor for Angiopoietin-1 (Ang1). Ang1 bridges TIE-2 at cell-cell contacts, resulting in trans-association of TIE-2 in the presence of cell-cell contacts. TIE-2 activation promotes vessel assembly and maturation by mediating survival signals for EC and regulating the recruitment of pericytes. TIE-2 maintains the vascular integrity of mature vessels by enhancing endothelial barrier function and inhibiting apoptosis of endothelial cells[1][2][3]. TIE-2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived TIE-2 protein, expressed by HEK293 , with C-His labeled tag. The total length of TIE-2 Protein, Mouse (HEK293, His) is 722 a.a., with molecular weight of 95-120 kDa.

研究背景

TIE-2 由 TEK 基因编码,是一种酪氨酸激酶受体,在血管稳定性中发挥核心作用。Ang1/Tie2 信号传导被认为可以调节血管静止的维持和血管生成的促进[1]
血管生成素 (Ang)/Tie2 系统是血管生物学的关键调节因子。膜结合 (mb) Tie2Ang-1 的表达可确保血管稳定性,而 Ang-2 可通过血管内皮生长因子 (VEGF)、缺氧和炎症诱导,充当拮抗剂。Tie2 信号传导也会被可溶性 Tie2 (sTie2) 减弱,sTie2 是受体的胞外结构域,在 VEGF 刺激后会脱落[2]

生物活性

1.The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
2.Immobilized Mouse Angiopoietin-2 at 4 μg/mL (100 μL/well) can bind Biotinylated TIE2. The ED50 for this effect is 460.2 ng/mL.

  • Immobilized Mouse Angiopoietin-2 at 4 μg/mL (100 μL/well) can bind Biotinylated TIE2. The ED50 for this effect is 460.2 ng/mL.
种属

Mouse

表达系统

HEK293

标签

C-His

蛋白编号

B1AWS8 (A23-K744)

基因 ID
蛋白结构
N-term
TIE-2 (A23-K744)
Accession # B1AWS8
His
C-term
同用名
Angiopoietin-1 receptor; CD202b; hTIE2; p140 TEK; Tie2; VMCM
氨基酸序列

AMDLILINSLPLVSDAETSLTCIASGWHPHEPITIGRDFEALMNQHQDPLEVTQDVTREWAKKVVWKREKASKINGAYFCEGRVRGQAIRIRTMKMRQQASFLPATLTMTVDRGDNVNISFKKVLIKEEDAVIYKNGSFIHSVPRHEVPDILEVHLPHAQPQDAGVYSARYIGGNLFTSAFTRLIVRRCEAQKWGPDCSRPCTTCKNNGVCHEDTGECICPPGFMGRTCEKACEPHTFGRTCKERCSGPEGCKSYVFCLPDPYGCSCATGWRGLQCNEACPSGYYGPDCKLRCHCTNEEICDRFQGCLCSQGWQGLQCEKEGRPRMTPQIEDLPDHIEVNSGKFNPICKASGWPLPTSEEMTLVKPDGTVLQPNDFNYTDRFSVAIFTVNRVLPPDSGVWVCSVNTVAGMVEKPFNISVKVLPEPLHAPNVIDTGHNFAIINISSEPYFGDGPIKSKKLFYKPVNQAWKYIEVTNEIFTLNYLEPRTDYELCVQLARPGEGGEGHPGPVRRFTTASIGLPPPRGLSLLPKSQTALNLTWQPIFTNSEDEFYVEVERRSLQTTSDQQNIKVPGNLTSVLLSNLVPREQYTVRARVNTKAQGEWSEELRAWTLSDILPPQPENIKISNITDSTAMVSWTIVDGYSISSIIIRYKVQGKNEDQHIDVKIKNATVTQYQLKGLEPETTYHVDIFAENNIGSSNPAFSHELRTLPHSPASADLGGGK

分子量

95-120 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
TIE-2 Protein, Mouse (HEK293, His)
目录号:
HY-P73610
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