TIGIT 蛋白, Rat (HEK293, Fc)

T-cell immunoreceptor with Ig and ITIM domains (TIGIT) is a member of the PVR (poliovirus receptor) family of immunoglobin proteins, binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation or T cell dependent B cell responses by promoting the generation of mature immunoregulatory dendritic cells. TIGIT Protein, Rat (HEK293, Fc) is the recombinant rat-derived TIGIT protein, expressed by HEK293 , with C-hFc labeled tag.

具有 Ig 和 ITIM 结构域的 T 细胞免疫受体 (TIGIT) 是 PVR (脊髓灰质炎病毒受体) 免疫球蛋白家族的成员，包含一个免疫球蛋白可变结构域、一个跨膜结构域和一个基于酪氨酸的免疫受体抑制基序。TIGIT 与 PVR 高亲和力结合，导致 IL10 分泌增加，IL12B 分泌减少，并通过促进成熟免疫调节性树突状细胞的产生，抑制 T 细胞活化或 T 细胞依赖性 B 细胞反应。TIGIT 在包括滤泡 B 辅助 T 细胞 (TFH) 在内的几种 T 细胞上表达^[1]。

Measured by its binding ability in a functional ELISA. Immobilized human Nectin-2 at 5 μg/mL (100 μL/well) can bind Biotinylated Rat TIGIT. The ED₅₀ for this effect is 0.04812 μg/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized human Nectin-2 at 5 μg/mL (100 μL/well) can bind Biotinylated Rat TIGIT. The ED₅₀ for this effect is 0.04812 μg/mL.

Rat

HEK293

C-hFc

XP_008766987 (A17-A137)

363784  [NCBI]

AFLAAGATAGTMETKGNISAEEGGSVVLQCHFSSDTAEVTQVNWEQRDQLLAVYSVDLGWYVPSVFSDRVVPGPSLGLTFQSLTTNDTGEYFCTYHTYPDGIYKGRIFLKVQESSAHFQIA

Approximately 48-58 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.