TIM-3/HAVCR2 蛋白, Cynomolgus (HEK293, His)

The TIM-3/HAVCR2 protein is a cell surface receptor that regulates immune responses by inhibiting macrophage activation and suppressing Th1-mediated autoimmunity. TIM-3/HAVCR2 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived TIM-3/HAVCR2 protein, expressed by HEK293 , with N-8*His labeled tag. The total length of TIM-3/HAVCR2 Protein, Cynomolgus (HEK293, His) is 180 a.a., with molecular weight of 26-30 kDa.

TIM-3/HAVCR2 蛋白是一种细胞表面受体，在调节先天性和适应性免疫反应中发挥着关键作用。主要被认为具有抑制功能，所报告的刺激功能表明基于细胞背景和配体特异性的细微影响。它调节巨噬细胞活化并抑制 1 型辅助 T 淋巴细胞 (Th1) 介导的自身免疫反应和同种免疫反应，从而促进免疫耐受。在 CD8+ 细胞中，TIM-3 通过阻断 NF-kappaB 和 NFAT 启动子活性来减弱 TCR 诱导的信号传导，从而导致 IL-2 分泌减少。这种抑制功能被认为涉及其与 LCK 的关联，损害 TCR 亚基的磷酸化。相反，TIM-3 已被证明可以激活 T 细胞中 TCR 诱导的信号传导，可能涉及 ZAP70、LCP2、LCK 和 FYN。LGALS9 的受体 TIM-3 与 LGALS9 的结合被认为可以抑制 T 细胞反应，导致抗原特异性细胞凋亡。此外，TIM-3 可以识别凋亡细胞上的磷脂酰丝氨酸，介导其吞噬作用，并积极调节先天免疫反应，特别是在树突状细胞中。它还在抑制肿瘤浸润树突状细胞中核酸介导的先天免疫反应中发挥作用，并在 LPS 诱导的内毒素休克中负向调节 NK 细胞功能。与各种信号分子（包括 HMGB1、BAG6、PIK3R1、PIK3R2、FYN 和 ILF3）的相互作用进一步促进了 TIM-3 在免疫反应中的复杂调节功能。

Cynomolgus

HEK293

N-8*His

G7P6Q7 (S22-R201)

10214172

SEVEYIAEVGQNAYLPCSYTPAPPGNLVPVCWGKGACPVFDCSNVVLRTDNRDVNDRTSGRYWLKGDFHKGDVSLTIENVTLADSGVYCCRIQIPGIMNDEKHNVKLVVIKPAKVTPAPTLQRDLTSAFPRMLTTGEHGPAETQTPGSLPDVNLTVSNFFCELQIFTLTNELRDSGATIR

26-30 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.