TIM-3/HAVCR2 蛋白, Human (HEK293, His)

T cell immunoglobulin mucin-3 is an inhibitory receptor, and inhibits T cell functions. TIM-3 regulates immune responses in autoimmunity and cancer. TIM-3 has multiple different ligands: galectin 9, phosphatidylserine (PtdSer), CEACAM1 and HMGB1. The TIM-3-ligand axis is critical in the pathogenesis of numerous conditions, including autoimmune diseases, infections, cancers, transplant rejection, and chronic inflammation^{[1][2][3][4][5]}. TIM-3/HAVCR2 Protein, Human (HEK293, His) is the recombinant human-derived TIM-3/HAVCR2 protein, expressed by HEK293 , with C-6*His labeled tag.

T 细胞免疫球蛋白 mucin-3 (TIM-3) 属于 Ig 超家族。TIM-3 通常由多种小鼠和人类免疫细胞类型表达。TIM-3 首次在产生 IFN-γ 的 Th1 和 Tc1 细胞上发现。TIM-3 作为抑制性受体，抑制 T 细胞功能。TIM-3 与自身免疫和癌症中免疫反应的调节有关^[1][5]。
TIM-3 具有多种不同的配体：半乳糖凝集素 9、磷脂酰丝氨酸 (PtdSer)、CEACAM1 和 HMGB1，这些配体与 TIM3 胞外免疫球蛋白 V 结构域上的不同区域结合。TIM-3-配体信号轴在多种疾病的发病机制中至关重要，包括自身免疫性疾病、感染、癌症、移植排斥和慢性炎症。例如，TIM-3 与 galectin-9 结合可以下调 Th1 反应^[2][3][4]。此外，Tim-3 表达失调与自身免疫性疾病相关^[5]。

1.Measured by its binding ability in a functional ELISA. Immobilized Human Galectin 9-His at 10 μg/mL (100 μl/well) can bind Human TIM-3-His*: Biotinylated by NHS-biotin prior to testing and the EC₅₀for the effect is 10.5 μg/mL.
2.Immobilized Anti-Human TIM-3 mAb at 2 μg/mL (100 μl/well) can bind TIM-3/HAVCR2, Human (HEK293, His). The ED₅₀ of TIM-3/HAVCR2, Human (HEK293, His) is 5-25 ng/mL.

Human

HEK293

C-6*His

AAL65157.1 (S22-R200)

84868  [NCBI]

SEVEYRAEVGQNAYLPCFYTPAAPGNLVPVCWGKGACPVFECGNVVLRTDERDVNYWTSRYWLNGDFRKGDVSLTIENVTLADSGIYCCRIQIPGIMNDEKFNLKLVIKPAKVTPAPTLQRDFTAAFPRMLTTRGHGPAETQTLGSLPDINLTQISTLANELRDSRLANDLRDSGATIR

Approximately 40 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.