TIMP-1 蛋白, Human (HEK293)

The TIMP-1 protein forms a one-to-one complex with the target metalloprotease, irreversibly inactivating it by binding to the catalytic zinc cofactor. It inhibits multiple MMPs, activates cell signaling through CD63 and ITGB1, and interacts with MMP1, MMP3, MMP10, and MMP13. TIMP-1 Protein, Human (HEK293) is the recombinant human-derived TIMP-1 protein, expressed by HEK293 , with tag free. The total length of TIMP-1 Protein, Human (HEK293) is 184 a.a., with molecular weight of ~26 kDa.

TIMP-1 是一种金属蛋白酶抑制剂，通过与靶金属蛋白酶（包括胶原酶）形成一对一复合物来发挥作用，通过与催化锌辅因子结合导致其不可逆失活。其抑制谱包括 MMP1、MMP2、MMP3、MMP7、MMP8、MMP9、MMP10、MMP11、MMP12、MMP13 和 MMP16，不包括 MMP14。除了作为酶抑制剂的作用外，TIMP-1 还充当生长因子，协调细胞分化、迁移和细胞死亡，同时通过与 CD63 和 ITGB1 相互作用激活复杂的细胞信号级联。这种多面蛋白还在整合素信号传导中发挥着至关重要的作用，尤其是在体外介导红细胞生成，与 IL3 不同，对人类和小鼠红细胞祖细胞表现出物种特异性刺激。此外，TIMP-1 参与与 MMP1、MMP3、MMP10 和 MMP13 的蛋白质-蛋白质相互作用，证明其对这些金属蛋白酶的调节影响。它与 CD63 和 ITGB1 形成复合物，进一步强调其参与复杂的细胞信号网络。

TIMP-1 (12.5-400 ng/mL；20 min) 增加 THP-1 单核细胞中 AKT 和 ERK1/2 的磷酸化水平。此外，TIMP-1 (100 ng/mL) 还能促进 THP-1 细胞中丝氨酸/苏氨酸激酶的激活^[1]。
TIMP-1 (50-200 ng/mL；1 h) 还能刺激 THP 细胞而介导细胞迁移和 CD74 内化，最佳浓度为 100 ng/mL^[1]。

Measured by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate MCA-PLGL-DPA-AR-NH2 and the IC₅₀ value is < 6 nM.

Human

HEK293

Tag Free

P01033 (C24-A207)

7076  [NCBI]

Approximately 26 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM NaAC, 200 mM NaCl, pH 5.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Ebert S, et al. Tissue Inhibitor of Metalloproteinases-1 Interacts with CD74 to Promote AKT Signaling, Monocyte Recruitment Responses, and Vascular Smooth Muscle Cell Proliferation. Cells. 2023 Jul 20;12(14):1899.  [Content Brief]