1. 重组蛋白
  2. Enzymes & Regulators
  3. Protease Inhibitors
  4. Tissue Inhibitor of Metalloproteinase (TIMPs)
  5. TIMP-1
  6. TIMP-1 蛋白, Mouse (HEK293)

TIMP-1 蛋白, Mouse (HEK293)

目录号: HY-P71110
COA 产品使用指南

TIMP-1 是一种金属蛋白酶抑制剂,通过与胶原酶和其他金属蛋白酶的催化锌辅因子结合,不可逆地灭活胶原酶和其他金属蛋白酶。它调节 MMP1、MMP2、MMP3、MMP7、MMP8、MMP9、MMP10、MMP11、MMP12、MMP13 和 MMP16,并影响分化、迁移和细胞死亡等细胞过程。TIMP-1 蛋白, Mouse (HEK293) 是重组的 TIMP-1 蛋白,由 HEK293 表达,不带标签。

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     可免费申领三个不同产品的试用装。

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规格 价格 是否有货 数量
10 μg ¥1700 In-stock
50 μg ¥4800 In-stock
100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

TIMP-1 is a metalloproteinase inhibitor that irreversibly inactivates collagenase and other metalloproteinases by binding to their catalytic zinc cofactor.It regulates MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16 and affects cellular processes such as differentiation, migration and cell death.TIMP-1 Protein, Mouse (HEK293) is the recombinant mouse-derived TIMP-1 protein, expressed by HEK293 , with tag free.

研究背景

TIMP-1 是一种金属蛋白酶抑制剂,通过与目标金属蛋白酶(例如胶原酶)形成一对一复合物来发挥其调节功能,通过与其催化锌辅因子结合导致这些酶不可逆失活。这种抑制作用涵盖一系列金属蛋白酶,包括 MMP1、MMP2、MMP3、MMP7、MMP8、MMP9、MMP10、MMP11、MMP12、MMP13 和 MMP16,但未观察到对 MMP14 的影响。除了作为酶抑制剂的作用外,TIMP-1 还作为生长因子,影响多种细胞过程,如分化、迁移和细胞死亡。它通过与 CD63 和 ITGB1 相互作用激活信号级联,表明它参与整合素信号传导。TIMP-1 还参与与 MMP1、MMP3、MMP10 和 MMP13 的蛋白质-蛋白质相互作用,证明其对这些金属蛋白酶的调节影响。此外,它与 CD63 和 ITGB1 形成复合物,表明它参与复杂的细胞信号网络。

生物活性

Measured by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2. The IC50 value is 0.131 nM, as measured under the described conditions.

种属

Mouse

表达系统

HEK293

标签

Tag Free

蛋白编号

P12032 (C25-R205)

基因 ID
蛋白结构
N-term
TIMP-1 (C25-R205)
Accession # P12032
C-term
同用名
Metalloproteinase Inhibitor 1; Erythroid-Potentiating Activity; EPA; Fibroblast collagenase Inhibitor; Collagenase Inhibitor; Tissue Inhibitor of Metalloproteinases 1; TIMP-1; TIMP1; CLGI; TIMP
氨基酸序列

CSCAPPHPQTAFCNSDLVIRAKFMGSPEINETTLYQRYKIKMTKMLKGFKAVGNAADIRYAYTPVMESLCGYAHKSQNRSEEFLITGRLRNGNLHISACSFLVPWRTLSPAQQRAFSKTYSAGCGVCTVFPCLSIPCKLESDTHCLWTDQVLVGSEDYQSRHFACLPRNPGLCTWRSLGAR

分子量

Approximately 26.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
TIMP-1 Protein, Mouse (HEK293)
目录号:
HY-P71110
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