Tissue Factor 蛋白, Human (HEK293, Fc)

Tissue Factor Protein, Human (HEK293, Fc) is a recombinant human CD142 expressed in HEK 293 cells with a Fc tag at the C-terminus. Coagulation Factor III/CD142 Protein is a principal regulator of oncogenic neoangiogenesis and controls therefore the cancerous process.

组织因子 (TF)，也称为 CD142，是一种 47 kDa 跨膜糖蛋白，是致癌性新血管生成的主要调节因子，因此控制癌变过程。TF 是唯一的膜结合凝血因子（因子 III）、辅因子和凝血因子 VII (fVII)/激活的 fVII (fVIIa) 的表面受体。TF通常由多个肿瘤区室选择性表达，包括但不限于癌细胞、癌症干细胞和肿瘤血管内皮细胞，而其在正常细胞中负表达、最低限度或限制性表达。TF 调节肿瘤生长、胚胎和致癌血管形成、炎症和败血症。

Human

HEK293

C-hFc

P13726-1 (S33-G251)

2152  [NCBI]

SGTTNTVAAYNLTWKSTNFKTILEWEPKPVNQVYTVQISTKSGDWKSKCFYTTDTECDLTDEIVKDVKQTYLARVFSYPAGNVESTGSAGEPLYENSPEFTPYLETNLGQPTIQSFEQVGTKVNVTVEDERTLVRRNNTFLSLRDVFGKDLIYTLYYWKSSSSGKKTAKTNTNEFLIDVDKGENYCFSVQAVIPSRTVNRKSTDSPVECMGQEKGEFRE

65-72 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<0.1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.