2. 重组蛋白
  3. Enzymes & Regulators
  4. Transferases (EC 2)
  5. TKT 蛋白, Human

TKT 蛋白, Human

目录号: HY-P701864
产品使用指南

TKT 蛋白对于催化二碳酮醇基团从酮糖供体到醛糖受体的转移至关重要。该过程通过与辅因子焦磷酸硫胺素形成共价中间体来促进。TKT 蛋白, Human 是重组的 TKT 蛋白，由 E. coli 表达，不带标签。TKT 蛋白, Human 全长 622 个氨基酸。

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1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 是否有货
50 μg   询价  
100 μg   询价  

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描述

TKT proteins are essential for catalyzing the transfer of a two-carbon ketol group from a ketose donor to an aldose acceptor. This process is facilitated by the formation of a covalent intermediate with the cofactor thiamine pyrophosphate. TKT Protein, Human is the recombinant human-derived TKT protein, expressed by E. coli , with tag free. The total length of TKT Protein, Human is 622 a.a., .
研究背景

TKT 蛋白对于催化二碳酮醇基团从酮糖供体向醛糖受体的转移至关重要。该过程通过与辅因子焦磷酸硫胺素形成共价中间体来促进。TKT 蛋白的酶活性在多种代谢途径中发挥着关键作用，包括磷酸戊糖途径和卡尔文循环。酮醇基团的转移是这些途径中的关键步骤，能够实现糖的转化以及能量生产和生物合成的重要中间体的生成。TKT 蛋白的功能对于维持代谢通量和促进细胞内碳水化合物代谢的整体平衡至关重要。
种属

Human
表达系统

E. coli
标签

Tag Free
蛋白编号

P29401 (E2-A623)
基因 ID

7086
蛋白结构
N-term
TKT (E2-A623)
Accession # P29401
C-term
同用名
TKT; Transketolase; TK
纯度

Greater than 85% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

TKT Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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