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  4. TLK2 蛋白, Human (749a.a, sf9, His, GST)

TLK2 蛋白, Human (749a.a, sf9, His, GST)

目录号: HY-P702737
产品使用指南

TLK2 是一种重要的丝氨酸/苏氨酸蛋白激酶,对染色质组装、DNA 复制、转录、修复和染色体分离具有复杂的影响。它通过磷酸化和稳定 ASF1A 和 ASF1B 等染色质组装因子来发挥多种作用,防止蛋白酶体介导的降解。TLK2 蛋白, Human (749a.a, sf9, His, GST) 是重组的 TLK2 蛋白,由 Sf9 insect cells 表达,带有 His, GST 标签。TLK2 蛋白, Human (749a.a, sf9, His, GST)

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描述

TLK2 is an important serine/threonine protein kinase with complex effects on chromatin assembly, DNA replication, transcription, repair, and chromosome segregation. It plays multiple roles by phosphorylating and stabilizing chromatin assembly factors such as ASF1A and ASF1B, preventing proteasome-mediated degradation. TLK2 Protein, Human (749a.a, sf9, His, GST) is the recombinant human-derived TLK2, expressed by Sf9 insect cells , with His, GST labeled tag. ,

研究背景

TLK2 是一种丝氨酸/苏氨酸蛋白激酶,在染色质组装的复杂过程中发挥着至关重要的作用,可能会将其影响扩展到 DNA 复制、转录、修复和染色体分离。该激酶通过磷酸化染色质组装因子(包括 ASF1A 和 ASF1B)并促进其稳定,特别是通过防止蛋白酶体介导的降解来发挥多方面的作用。通过增强染色质组装,TLK2 积极参与基因组完整性的维护。此外,TLK2 作为氨基酸饥饿诱导的自噬的负调节因子发挥作用,表明它参与细胞对营养信号的反应。这种多功能激酶对基本细胞过程具有影响,在核和细胞动力学各个方面的复杂协调中发挥着关键作用。

种属

Human

表达系统

Sf9 insect cells

标签

N-8*His;N-GST

蛋白编号

Q86UE8-1 (M2-S750)

基因 ID

11011

同用名
TLK2; tousled-like kinase 2; serine/threonine-protein kinase tousled-like 2; MGC44450; PKU ALPHA; serine/threonine kinase; PKU-ALPHA
纯度

Greater than 80 % as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

TLK2 Protein, Human (749a.a, sf9, His, GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
TLK2 Protein, Human (749a.a, sf9, His, GST)
目录号:
HY-P702737
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