TNF-alpha/TNFSF2 蛋白, Guinea (N-His)

TNF-alpha/TNFSF2 protein binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR, inducing cell death in specific tumors and causing fever. It can also stimulate cell proliferation, induce insulin resistance, promote angiogenesis, and mediate bone resorption. TNF-alpha's intracellular domain induces IL12 production, highlighting its diverse physiological impact. TNF-alpha/TNFSF2 Protein, Guinea (N-His) is the recombinant TNF-alpha/TNFSF2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of TNF-alpha/TNFSF2 Protein, Guinea (N-His) is 156 a.a., with molecular weight of ~19 kDa.

TNF-α/TNFSF2 蛋白是一种细胞因子，可与 TNFRSF1A/TNFR1 和 TNFRSF1B/TNFBR 结合。它主要由巨噬细胞分泌，具有诱导特定肿瘤细胞系细胞死亡的能力，并作为强热原，通过直接作用或刺激白介素-1 分泌而引起发烧。TNF-α参与恶病质的诱导，并且在某些条件下，它可以刺激细胞增殖并诱导细胞分化。此外，它通过抑制胰岛素诱导的 IRS1 酪氨酸磷酸化和胰岛素诱导的葡萄糖摄取来诱导脂肪细胞中的胰岛素抵抗，从而导致脂肪细胞中的 GKAP42 蛋白降解，从而导致 TNF 诱导的胰岛素抵抗。除了其代谢作用之外，TNF-α 通过与 IL1B 和 IL6 协同诱导 VEGF 产生，在血管生成中发挥作用。此外，它还能促进破骨细胞生成，介导骨吸收，其细胞内结构域 (ICD) 形式可诱导树突状细胞产生 IL12，强调其对多种生理过程的多方面影响。

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ this effect is 0.04334 ng/mL, corresponding to a specific activity is 2.307×10⁷units/mg.

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  Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is 0.04334 ng/mL, corresponding to a specific activity is 2.307×10⁷ units/mg.

Others

E. coli

N-6*His

P51435 (L79-L234)

100135630  [NCBI]

LRSASQNDNDKPVAHVVANQQAEEELQWLSKRANALLANGMGLSDNQLVVPSDGLYLIYSQVLFKGQGCPSYLLLTHTVSRLAVSYPEKVNLLSAIKSPCQKETPEGAERKPWYEPIYLGGVFQLQKGDRLSAEVNLPQYLDFADSGQIYFGVIAL

Approximately 19 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.