1. 重组蛋白
  2. Cytokines and Growth Factors
  3. TNF Superfamily Neurotrophic Factors
  4. TNF Superfamily Ligands
  5. TNF-alpha
  6. TNF-alpha/TNFSF2 蛋白, Marmota monax (Baculovirus, His)

TNF-alpha/TNFSF2 蛋白, Marmota monax (Baculovirus, His)

目录号: HY-P700504
产品使用指南

TNF-α/TNFSF2 蛋白是巨噬细胞分泌的细胞因子,可结合 TNFRSF1A/TNFR1 和 TNFRSF1B/TNFBR。它诱导肿瘤细胞死亡,作为强热原引起发烧,并与恶病质诱导有关。TNF-alpha/TNFSF2 蛋白, Marmota monax (Baculovirus, His) 是重组的 TNF-α/TNFSF2 蛋白,由 Sf9 insect cells 表达,带有 N-10*His 标签。TNF-alpha/TNFSF2 蛋白, Marmota monax (Baculovirus, His) 全长 177 个氨基酸,分子量为 22.2 kDa。

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描述

TNF-α/TNFSF2 protein is a cytokine secreted by macrophages that binds TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It induces tumor cell death, acts as a potent pyrogen causing fever, and is associated with cachexia induction. TNF-alpha/TNFSF2 Protein, Marmota monax (Baculovirus, His) is the recombinant TNF-alpha/TNFSF2 protein, expressed by Sf9 insect cells , with N-10*His labeled tag. The total length of TNF-alpha/TNFSF2 Protein, Marmota monax (Baculovirus, His) is 177 a.a., with molecular weight of 22.2 kDa.

研究背景

TNF-α/TNFSF2 蛋白是一种细胞因子,可与 TNFRSF1A/TNFR1 和 TNFRSF1B/TNFBR 结合。它主要由巨噬细胞分泌,具有诱导特定肿瘤细胞系细胞死亡的能力,并作为强热原发挥作用,通过直接作用或刺激白介素-1 分泌而引起发热。TNF-α 与恶病质的诱发有关,具有多种作用;在一定条件下,它可以刺激细胞增殖并诱导细胞分化。此外,它还通过抑制胰岛素诱导的 IRS1 酪氨酸磷酸化和胰岛素诱导的葡萄糖摄取来诱导脂肪细胞中的胰岛素抵抗,从而导致 GKAP42 蛋白降解和 TNF 诱导的胰岛素抵抗。TNF-α 通过与 IL1B 和 IL6 协同诱导 VEGF 产生,在血管生成中发挥作用,并促进破骨细胞生成,介导骨吸收。TNF-α 的胞内结构域 (ICD) 形式可诱导树突状细胞产生 IL12,突显其对各种生理过程的多方面影响。

种属

Others

表达系统

Sf9 insect cells

标签

N-10*His

蛋白编号

O35734 (G57-L233)

基因 ID

124109351  [NCBI]

蛋白结构
N-term
10*His
TGF-α (G57-L233)
Accession # O35734
C-term
同用名
rHuTNF-α, His; Cachectin; TNFSF2
氨基酸序列

GPQREEFLNNLPLSPQAQMLTLRSSSQNMNDKPVAHVVAKNEDKEQLVWLSRRANALLANGMELIDNQLVVPANGLYLVYSQVLFKGQGCPSYVLLTHTVSRFAVSYQDKVNLLSAIKSPCPKESLEGAEFKPWYEPIYLGGVFELQKGDRLSAEVNLPSYLDFAESGQVYFGVIAL

分子量

22.2 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
TNF-alpha/TNFSF2 Protein, Marmota monax (Baculovirus, His)
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HY-P700504
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