TNF-alpha/TNFSF2 蛋白, Pig (N-His)

TNF-α/TNFSF2 protein is a cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and has multiple biological functions. It has the ability to induce cell death in specific tumor cell lines, serves as a potent pyrogen, can cause fever through direct action or stimulation of interleukin-1 secretion, and is involved in the induction of cachexia. TNF-alpha/TNFSF2 Protein, Pig (N-His) is the recombinant Pig-derived TNF-alpha/TNFSF2 protein, expressed by E. coli , with N-6*His labeled tag.

TNF-α/TNFSF2 蛋白是一种细胞因子，可与 TNFRSF1A/TNFR1 和 TNFRSF1B/TNFBR 结合，主要由巨噬细胞分泌，具有多种生物学功能。它具有诱导特定肿瘤细胞系细胞死亡的能力，作为一种有效的热原，可以通过直接作用或刺激白细胞介素-1 分泌而引起发烧，并参与恶病质的诱导。在某些条件下，TNF-α/TNFSF2 可以发挥刺激细胞增殖和诱导细胞分化的作用。它还通过抑制胰岛素诱导的 IRS1 酪氨酸磷酸化和葡萄糖摄取来促进脂肪细胞中的胰岛素抵抗，并对 GKAP42 蛋白降解产生额外影响。此外，TNF-α/TNFSF2 通过与 IL1B 和 IL6 协同诱导 VEGF 产生来参与血管生成，并促进破骨细胞生成，从而介导骨吸收。TNF-α 的胞内结构域 (ICD) 形式可诱导树突状细胞产生 IL12，进一步凸显其对不同细胞过程的多方面影响。

Measured in a cytotoxicity assay using TNF-susceptible PK-15 porcine kidney epithelial cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 0.06386 ng/mL, corresponding to a specific activity is 1.57×10^7 units/mg.

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  Measured in a cytotoxicity assay using TNF-susceptible PK-15 porcine kidney epithelial cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is 0.06386 ng/mL，corresponding to a specific activity is 1.57×10⁷ units/mg.

Pig

E. coli

N-6*His

P23563 (R78-L232)

397086

RSSSQTSDKPVAHVVANVKAEGQLQWQSGYANALLANGVKLKDNQLVVPTDGLYLIYSQVLFRGQGCPSTNVFLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKDDRLSAEINLPDYLDFAESGQVYFGIIAL

approximately 20 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.