TNFRII 蛋白, Human (HEK293, hFc)

TNFRII (TNFRSF1B) protein has a high ability to bind with tumor necrosis factor-alpha (TNF-α). TNFRII has pro-apoptotic function. TNFRII recruits TRAF2, induces gene expression and intensively crosstalks with TNF-R1. TNFRII selectively enhances the induction of apoptosis by the death receptor TNFRI. TNFRII Protein, Human (HEK293, hFc) is expressed by HEK293 and has a transmembrane region (L23-D257) with hFc tag at the C-terminus^[1].

TNFRII (TNFRSF1B) 蛋白是一种单通道 I 型膜蛋白，属于肿瘤坏死因子 (TNF) 家族。TNFRII 是 TNF-α 的主要信号受体。TNFRII 蛋白受到高度调控，通常存在于免疫系统细胞中^[1]。
小鼠 TNFRII 蛋白的氨基酸序列与人类和恒河猴 TNFRII 蛋白的同源性较低（低于 85%） ）。人和恒河猴的 TNFRII 蛋白氨基酸序列非常相似（同一性矩阵百分比为 95.88%）。
TNFRII 诱导细胞凋亡。TNFRII 不直接参与细胞凋亡程序，而是依赖于内源性膜结合 TNF 的诱导，随后激活 TNFRI。TNFRII刺激内源性产生的膜结合TNF对TNFRI的作用显着增强。TNFRII 与 TNFRI 竞争招募新合成的 TRAF2 结合抗凋亡因子，从而促进 caspase-8 激活 TNFRI 复合物的形成。TNFRII 与 TNFRI 竞争结合 TRAF2 和 TRAF2 相关的抗凋亡 cIAP1 和 cIAP2 蛋白。cIAP1 启动 TRAF2 的降解，进而增强受体对剩余 TRAF2、cIAP1 和 cIAP2 分子的竞争。cIAP1 在招募到 TNFRI 信号复合物后将具有抗凋亡功能，但在招募到 TNFRII 信号复合物后将转变为净促凋亡功能^[1][2][3]。

1.Measured by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect, in the presence of 0.25 ng/mL of TNF-alpha, is 0.0014 µg/mL corresponding to a specific activity is 7.143×10⁵ units/mg.
2.Measured by its binding ability in a functional ELISA. Immobilized TNF-α at 0.5 μg/ml can bind human TNFR2, the EC50 of human TNFR2 protein is 1.367 - 2.114 ng/ml.

Human

HEK293

C-hFc

P20333 (L23-D257)

7133  [NCBI]

LPAQVAFTPYAPEPGSTCRLREYYDQTAQMCCSKCSPGQHAKVFCTKTSDTVCDSCEDSTYTQLWNWVPECLSCGSRCSSDQVETQACTREQNRICTCRPGWYCALSKQEGCRLCAPLRKCRPGFGVARPGTETSDVVCKPCAPGTFSNTTSSTDICRPHQICNVVAIPGNASMDAVCTSTSPTRSMAPGAVHLPQPVSTRSQHTQPTPEPSTAPSTSFLLPMGPSPPAEGSTGD

Approximately 68 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Wajant H, et, al. Tumor necrosis factor signaling. Cell Death Differ. 2003 Jan;10(1):45-65.  [Content Brief]

  [2]. Fotin-Mleczek M, et, al. Apoptotic crosstalk of TNF receptors: TNF-R2-induces depletion of TRAF2 and IAP proteins and accelerates TNF-R1-dependent activation of caspase-8. J Cell Sci. 2002 Jul 1;115(Pt 13):2757-70.  [Content Brief]

  [3]. Masli S, et, al. Anti-inflammatory effects of tumour necrosis factor (TNF)-alpha are mediated via TNF-R2 (p75) in tolerogenic transforming growth factor-beta-treated antigen-presenting cells. Immunology. 2009 May;127(1):62-72.  [Content Brief]