TNFRSF1A 蛋白, Rat (HEK293, His)

TNFRSF1A (TNF RI) protein has a high ability to bind with tumor necrosis factor-alpha (TNF-α). TNFRSF1A is a STAT3 target gene that regulates the NF-κB pathway. TNFRSF1A activate NF-κB, mediate apoptosis, and function as a regulator of inflammation. TNFRSF1A Protein, Rat (HEK293, His) is expressed by HEK293 and has a transmembrane region (M1-A211) with a His tag at the C-terminus^[1].

TNFRSF1A (TNF RI) 蛋白是一种单通道 I 型膜蛋白，属于肿瘤坏死因子 (TNF) 家族。TNFRSF1A 是 TNF-α 的主要信号受体。TNFRSF1A蛋白是一种多功能细胞因子，几乎所有细胞均合成该蛋白^[1][2]。
不同物种TNFRSF1A的氨基酸序列为非常不同（人类、大鼠和小鼠之间的相似性低于 85%）。
TNFRSF1A 包含一个蛋白质-蛋白质相互作用结构域，称为死亡结构域 (DD)，可以招募其他含有 DD 的蛋白质并将死亡受体与 caspase 偶联激活和凋亡。可溶性和膜结合形式的细胞因子均可激活 TNFRSF1A。TNFRSF1A 通过参与 mTOR、JNK、IKK、caspase 3、MAPK 和 NF-κB 通路诱导细胞炎症损伤和细胞凋亡^[1][3][4]。

Measured by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is 26.87 ng/mL in the presence of 0.1 ng/mL of recombinant human TNF-alpha, corresponding to a specific activity is 3.72×10⁴ units/mg.

Rat

HEK293

C-His

P22934/NP_037223.1 (L30-A211)

25625  [NCBI]

LVPSLGDREKRDNLCPQGKYAHPKNNSICCTKCHKGTYLVSDCPSPGQETVCEVCDKGTFTASQNHVRQCLSCKTCRKEMFQVEISPCKADMDTVCGCKKNQFQRYLSETHFQCVDCSPCFNGTVTIPCKEKQNTVCNCHAGFFLSGNECTPCSHCKKNQECMKLCLPPVANVTNPQDSGTA

Approximately 30-40 kDa due to glycosylation

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Wajant H, et, al. Tumor necrosis factor signaling. Cell Death Differ. 2003 Jan;10(1):45-65.  [Content Brief]

  [2]. Fu Q, et, al. miR-29a up-regulation in AR42J cells contributes to apoptosis via targeting TNFRSF1A gene. World J Gastroenterol. 2016 May 28;22(20):4881-90.  [Content Brief]

  [3]. Zhou S, et, al. Bioinformatics Analysis Identifies TNFRSF1A as a Biomarker of Liver Injury in Sepsis TNFRSF1A is a Biomarker for Septic Liver Injury. Genet Res (Camb). 2022 Oct 15;2022:1493744.  [Content Brief]

  [4]. Egusquiaguirre SP, et, al. The STAT3 Target Gene TNFRSF1A Modulates the NF-κB Pathway in Breast Cancer Cells. Neoplasia. 2018 May;20(5):489-498.  [Content Brief]