TNFRSF3/LTBR 蛋白, Rat (HEK293, His)

TNFRSF3/LTBR Protein, Rat (HEK293, His) is a cell surface receptor for apoptotic and cytokine-released lymphotoxins involved in activation of gene transcription programs and cell death, and is important in immune development and host defense. TNFRSF3/LTBR Protein, Rat (HEK293, His) is expressed by HEK293 cells and is a transmembrane protein (M222-W245) with a His tag at the C-terminus^[1].

淋巴毒素β受体（LTBR），也称为肿瘤坏死因子受体超家族成员3（TNFRSF3），是肿瘤坏死因子受体超家族的成员，也是参与细胞凋亡和细胞因子释放的淋巴毒素的细胞表面受体。LTBR在大多数细胞类型的表面表达，包括乳腺癌、结直肠癌、肺癌、胃癌、黑色素瘤和膀胱癌，而其配体淋巴毒素（LT）a1b2和TNF超家族成员14（TNFSF14；也称为LIGHT）主要是表达于免疫细胞表面。LTBR信号通路可能参与激活控制细胞分化、生长和死亡的反应，如外周淋巴样器官的形成所证明的，特别是对组织、树突状细胞稳态、肝脏再生至关重要的二级和三级淋巴结构。干扰素对病原体的反应和粘膜源性癌的死亡。LTβR 信号传导可能促进浸润免疫细胞和肿瘤细胞之间的通讯。触发 LTβR 会诱导典型和非典型核因子 (NF)-κB 信号通路，这些信号通路与炎症诱导的致癌作用相关。持续的 LTβR 信号传导还会导致 NF-κB 介导的慢性炎症和肝细胞癌 (HCC)^[1][2]。

Measured by its binding ability in a functional ELISA. Immobilized Rat TNFRSF3 at 5 μg/mL (100 μL/well) can bind biotinylated Human LIGHT. The ED₅₀ for this effect is 35.04 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Rat TNFRSF3 at 1 μg/mL (100 μL/well) can bind biotinylated Human LIGHT .The ED₅₀ for this effect is 86.95 ng/mL.

Rat

HEK293

C-His

Q5U2S8 (S28-A218)

297604  [NCBI]

SQPQLVPPYRIENQTCWDPDKEYYEPLHQVCCSRCPPGKFVHAVCSPSQDTVCKTCLHNSYNEHWNHLFSCQLCRPCDSVLGFEEIAPCTSDRKPECRCKPGMSCVYLDNECVHCEEERLVLCRPGTEAEVTDEIMDTEVNCVPCKPGHFQNTSSPRARCQPHTRCESQGLVEAASGTSYSDTICKNPPEA

Approximately 30-37 kDa due to glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Norris PS, et al. The LT beta R signaling pathway. Adv Exp Med Biol. 2007;597:160-72.  [Content Brief]

  [2]. Mo Shen, et al. Lymphotoxin β receptor activation promotes mRNA expression of RelA and pro-inflammatory cytokines TNFα and IL-1β in bladder cancer cells. Mol Med Rep. 2017 Jul;16(1):937-942.  [Content Brief]