TPO/Thrombopoietin 蛋白, Human (CHO, His)

TPO/Thrombopoietin Protein, Human (CHO)，also called megakaryocyte growth and development factor, is a cytokine that regulates megakaryocyte and platelet production.

重组血小板生成素可减少放射和化疗引起的血小板减少症，增强骨髓移植后的血小板恢复，并增加干细胞收获物中巨核细胞前体细胞的数量。向动物注射血小板生成素可刺激骨髓巨核细胞的数量、大小和倍性，并使血小板计数增加至十倍^[1]。超生理量的血小板生成素/TPO (>100 ng/mL) 能够在体外直接激活血小板聚集。血小板生成素/TPO 对血流下的血小板粘附也有显着影响。低血小板生成素/TPO 浓度 (001-1 ng/mL) 可加速血小板与血管性血友病因子的牢固粘附，并防止在较高流速下脱离^[2]。

The ED₅₀ is typically 0.5-5 ng/mL as measured by MO7e human megakaryocytic leukemic cells in a cell proliferation assay.

Human

CHO

C-His

P40225 (S22-G353)

7066  [NCBI]

MELTELLLVVMLLLTARLTLSSPAPPACDLRVLSKLLRDSHVLHSRLSQCPEVHPLPTPVLLPAVDFSLGEWKTQMEETKAQDILGAVTLLLEGVMAARGQLGPTCLSSLLGQLSGQVRLLLGALQSLLGTQLPPQGRTTAHKDPNAIFLSFQHLLRGKVRFLMLVGGSTLCVRRAPPTTAVPSRTSLVLTLNELPNRTSGLLETNFTASARTTGSGLLKWQQGFRAKIPGLLNQTSRSLDQIPGYLNRIHELLNGTRGLFPGPSRRTLGAPDISSGTSDTGSLPPNLQPGYSPSPTHPPTGQYTLFPLPPTLPTPVVQLHPLLPDPSAPTPTPTSPLLNTSYTHSQNLSQEG

Approximately 60-80 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Kuter DJ, et al. Thrombopoietin: Biology and Clinical Applications. Oncologist. 1996;1(1 & 2):98-106.  [Content Brief]

  [2]. Hitchcock IS, et al. Thrombopoietin from beginning to end. Br J Haematol. 2014 Apr;165(2):259-68.  [Content Brief]