TRAIL/TNFSF10 蛋白, Human (N-His)

TRAIL/TNFSF10 protein is a cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and possibly TNFRSF11B/OPG. It induces apoptosis, a process that can be mediated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4, and TNFRSF11B/OPG, which lack the ability to induce apoptosis. TRAIL/TNFSF10 Protein, Human (N-His) is the recombinant human-derived TRAIL/TNFSF10 protein, expressed by E. coli , with N-6*His labeled tag. The total length of TRAIL/TNFSF10 Protein, Human (N-His) is 168 a.a., with molecular weight of ~22 kDa.

TRAIL/TNFSF10 蛋白是一种细胞因子，可与 TNFRSF10A/TRAILR1、TNFRSF10B/TRAILR2、TNFRSF10C/TRAILR3、TNFRSF10D/TRAILR4 以及可能的 TNFRSF11B/OPG 结合。它诱导细胞凋亡，这一过程可以通过与诱饵受体 TNFRSF10C/TRAILR3、TNFRSF10D/TRAILR4 和 TNFRSF11B/OPG 结合来调节，而这些受体缺乏诱导细胞凋亡的能力。作为同源三聚体存在，一个 TNFSF10 同源三聚体与三个 TNFSF10A 单体相互作用，类似地，一个 TNFSF10 同源三聚体与三个 TNFSF10B 单体相互作用。这种复杂的相互作用强调了 TRAIL/TNFSF10 介导的细胞凋亡信号传导的复杂性，展示了其与多个受体结合并形成不同的分子构型以实现其生物活性的能力。

Measured in a cytotoxicity assay using L929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.The ED₅₀ for this effect is 5.448 ng/mL, corresponding to a specific activity is 1.8355×10⁵ units/mg.

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  Measured in a cytotoxicity assay using L929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.The ED₅₀ for this effect is 5.448 ng/mL, corresponding to a specific activity is 1.8355×10⁵ units/mg.

Human

E. coli

N-6*His

P50591 (V114-G281)

8743  [NCBI]

VRERGPQRVAAHITGTRGRSNTLSSPNSKNEKALGRKINSWESSRSGHSFLSNLHLRNGELVIHEKGFYYIYSQTYFRFQEEIKENTKNDKQMVQYIYKYTSYPDPILLMKSARNSCWSKDAEYGLYSIYQGGIFELKENDRIFVSVTNEHLIDMDHEASFFGAFLVG

Approximately 22 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.