TRAIL/TNFSF10 蛋白, Mouse (His)

TRAIL/TNFSF10 protein binds to TNFRSF10A, TNFRSF10B, TNFRSF10C, TNFRSF10D, and possibly TNFRSF11B, inducing apoptosis.It can be modulated by decoy receptors TNFRSF10C, TNFRSF10D, and TNFRSF11B, which do not induce apoptosis.TRAIL/TNFSF10 exists as a homotrimer, interacting with its receptor monomers.This complex molecular interaction governs apoptotic signaling pathways.TRAIL/TNFSF10 Protein, Mouse (His) is the recombinant mouse-derived TRAIL/TNFSF10 protein, expressed by E.coli , with N-6*His labeled tag.

TRAIL/TNFSF10 蛋白是一种细胞因子，可与 TNFRSF10A/TRAILR1、TNFRSF10B/TRAILR2、TNFRSF10C/TRAILR3、TNFRSF10D/TRAILR4 以及可能的 TNFRSF11B/OPG 结合，诱导细胞凋亡。其凋亡活性可通过与诱饵受体 TNFRSF10C/TRAILR3、TNFRSF10D/TRAILR4 和 TNFRSF11B/OPG 结合来调节，这些受体缺乏诱导凋亡的能力。TRAIL/TNFSF10 作为同源三聚体存在，其中一个 TRAIL/TNFSF10 同源三聚体与三个 TNFRSF10A 单体相互作用，另一个同源三聚体与三个 TNFRSF10B 单体相互作用。这种多价相互作用强调了控制 TRAIL/TNFSF10 及其受体介导的细胞凋亡信号通路的复杂分子相互作用。

Measured in a cytotoxicity assay using L929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is 0.2378 ng/mL, corresponding to a specific activity is 4.205×10⁶ units/mg.

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  Measured in a cytotoxicity assay using L929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED₅₀ for this effect is 0.2378 ng/mL, corresponding to a specific activity is 4.205×10⁶ units/mg.

Mouse

E. coli

N-6*His

P50592 (P118-N291)

22035

PRGGRPQKVAAHITGITRRSNSALIPISKDGKTLGQKIESWESSRKGHSFLNHVLFRNGELVIEQEGLYYIYSQTYFRFQEAEDASKMVSKDKVRTKQLVQYIYKYTSYPDPIVLMKSARNSCWSRDAEYGLYSIYQGGLFELKKNDRIFVSVTNEHLMDLDQEASFFGAFLIN

Approximately 21.82 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.