1. 重组蛋白
  2. Enzymes & Regulators Ubiquitin Related Proteins
  3. Transferases (EC 2) Ubiquitin Enzymes
  4. E3 Ligases
  5. TRIM/RBCC Proteins
  6. TRIM69 蛋白, Human

TRIM69 蛋白, Human

目录号: HY-P701511
产品使用指南

TRIM69蛋白是一种E3泛素连接酶,通过泛素化登革热病毒NS3等病毒蛋白,从而限制病毒感染(如登革热病毒、印第安纳水泡性口炎病毒),从而限制感染。TRIM69 蛋白, Human 是重组的 TRIM69 蛋白,由 E. coli 表达,不带标签。TRIM69 蛋白, Human 全长 499 个氨基酸。

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描述

TRIM69 protein is an E3 ubiquitin ligase that limits viral infection (such as dengue virus, Indiana vesicular stomatitis virus) by ubiquitinating viral proteins such as dengue virus NS3, thereby limiting infection. TRIM69 Protein, Human is the recombinant human-derived TRIM69 protein, expressed by E. coli , with tag free. The total length of TRIM69 Protein, Human is 499 a.a., .

研究背景

TRIM69 作为 E3 泛素连接酶,通过限制登革热病毒和水泡性口炎印第安纳病毒等多种病毒感染,在抗病毒免疫中发挥关键作用。这种调节功能是通过病毒蛋白(例如登革热病毒 NS3)的泛素化来实现的,从而抑制感染的程度。除了其抗病毒作用之外,TRIM69 还作为 I 型干扰素诱导的微管稳定的中心介质,与微管建立了直接关联,独立于其 E3 连接酶活性。此外,它与 TP53 一起导致白内障形成。从机制上讲,TRIM69 通过诱导 TP53 泛素化来抑制 UVB 诱导的细胞凋亡和活性氧 (ROS) 产生,突显其在细胞过程中的多方面参与。

种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

Q86WT6 (E2-Q500)

基因 ID

140691

蛋白结构
N-term
TRIM69 (E2-Q500)
Accession # Q86WT6
C-term
同用名
TRIM69; E3 ubiquitin-protein ligase TRIM69; RFP-like domain-containing protein trimless; RING finger protein 36; RING-type E3 ubiquitin transferase TRIM69; Tripartite motif-containing protein 69
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
TRIM69 Protein, Human
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HY-P701511
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