TrkA 蛋白, Rabbit (HEK293, His)

TrkA Protein, Rabbit (HEK293, His) is a nerve growth factor (NGF) receptor that belongs to the tyrosine kinase receptor family. TRKA (NTRK1) gene encodes the high affinity receptor for a neurotrophin nerve growth factor (NGF). It is critical for the correct development of many types of neurons including pain-mediating sensory neurons and also controls proliferation, differentiation and survival of many neuronal and non-neuronal cells^[1][2]. TrkA Protein, Rabbit (HEK293, His) is the recombinant Rabbit-derived TrkA protein, expressed by HEK293 , with C-His labeled tag.

TRKA 与高度相似的受体 TRKB 和 TRKC 属于酪氨酸激酶受体组。TRKB 结合神经营养蛋白脑源性神经营养因子 (BDNF) 和神经营养蛋白-4 (NT-4)，而 TRKC 是神经营养蛋白 NT-3 的主要受体，尽管 TRKA 和 TRKB 也可以被 NT-3 激活。NGF-TRKA 复合物启动的信号传导对于介导疼痛的感觉神经元、节后交感神经元和基底前脑胆碱能神经元的发育至关重要^[1]。
TRKA (NTRK1) 基因是选择性剪接的靶标，可产生多种不同的蛋白质亚型。TRKA 由位于染色体 1q21-q22 上的 NTRK1 基因编码。目前，已描述了三种人类亚型 (TRKAI、TRKAII 和 TRKAIII) 和两种大鼠亚型 (TRKA L0 和 TRKA L1)。人类 TRKA 基因由两个基因重叠，跨度为 67 kb。大鼠和小鼠中的 TrkA 基因似乎相当短，不与其他基因重叠。人类 TRKA 基因位于 1 号染色体上，据描述跨度为 23 kb。与人类 TRKA 基因相比，大鼠和小鼠中相对保守的 17 个外显子已被表征，基本局部比对搜索工具 (BLAST) 算法在这两种情况下给出了 85% 的相似性^[1][2]。

Measured by its ability to inhibit NGF-induced proliferation of TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 0.9070 μg/mL in the presence of 10 ng/mL of NGF, corresponding to a specific activity is 1.103×10³ U/mg

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  Measured by its ability to inhibit NGF-induced proliferation of TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 0.9070 μg/mL in the presence of 10 ng/mL of NGF, corresponding to a specific activity is 1.103×10³ U/mg

Rabbit

HEK293

C-6*His

XP_008262512 (A33-E414)

100354292  [NCBI]

AALCPDVCCPRGPSGLLCTRPGALDRLRHLPGIENLTELYLENQNLQHLTLGDLRGLRELRNLAIVNSGLQSVATDAFRFTPRLSHLNLSFNALESLSWKTVQGLPLQELVLSGNSLRCSCALRWLQRWEEEGLAGVREQKLRCSESEPLALMPNASCGMPTLKVQMPNGSVDVGDSVFLQCQVEGQGLEKAGWSLTELEELATVMIQKSEDLPTLRLTLANVTSDLNRKNVTCWAENDVGRTEVSVQVNVSFPASVQLHTAVEMHHWCIPFSVDGQPAPSLHWLFNGSVLNETSFIFTEFLEPAANETMRHGCLRLNQPTHVNNGNYTLLATNPSGQAAASIMAAFMDNPFEFNPEDPIPVSFSPVDANSTSGDPVEKKDE

Approximately 45-100 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.