TrkB 蛋白, Rat (HEK293, C-His)

TrkB is a key receptor tyrosine kinase that coordinates multiple neuronal processes in the central and peripheral nervous systems.As a receptor for BDNF and NTF4, it regulates survival through NTF3.TrkB Protein, Rat (HEK293, C-His) is the recombinant rat-derived TrkB protein, expressed by HEK293 , with C-6*His labeled tag.

TrkB 是一种受体酪氨酸激酶，复杂地协调中枢和周围神经系统的发育和成熟，对各种神经元过程进行调节控制，包括生存、增殖、迁移、分化和突触形成。作为 BDNF/脑源性神经营养因子和 NTF4/神经营养蛋白-4 的受体，它可以选择性地结合 NTF3/神经营养蛋白-3，通过 NTRK2 调节神经元存活。配体结合后，TrkB 经历同二聚化、自磷酸化和激活，启动一系列下游事件。它招募、磷酸化并激活多种效应子，例如 SHC1、FRS2、SH2B1、SH2B2 和 PLCG1，这些效应器协调不同的信号传导途径，包括用于神经元分化的 GRB2-Ras-MAPK 级联、用于生长的 Ras-PI3 激酶-AKT1 途径和生存，以及 PLCG1 调节的突触可塑性途径。TrkB 参与学习和记忆包括短期突触功能和长期增强的调节。此外，通过 PLCG1，它还能激活 NF-Kappa-B 和生存相关基因的转录，从而抑制失巢凋亡（一种因细胞与基质相互作用丧失而导致的细胞凋亡形式）。值得注意的是，TrkB 还可能有助于神经胶质细胞中神经营养蛋白依赖性钙信号传导。

Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED₅₀ this effect is 0.3638 μg/mL, corresponding to a specific activity is 2.75×10^3 units/mg.

Rat

HEK293

C-6*His

Q63604-2 (C32-H429)

25054

CPMSCKCSTTRIWCTEPSPGIVAFPRLEPNSIDPENITEILIANQKRLEIINEDDVEAYVGLKNLTIVDSGLKFVAYKAFLKNGNLRHINFTRNKLTSLSRRHFRHLDLSDLILTGNPFTCSCDIMWLKTLQETKSSPDTQDLYCLNESSKNTPLANLQIPNCGLPSARLAAPNLTVEEGKSVTISCSVGGDPLPTLYWDVGNLVSKHMNETSHTQGSLRITNISSDDSGKQISCVAENLVGEDQDSVNLTVHFAPTITFLESPTSDHHWCIPFTVRGNPKPALQWFYNGAILNESKYICTKIHVTNHTEYHGCLQLDNPTHMNNGDYTLMAKNEYGKDERQISAHFMGRPGVDYETNPNYPEVLYEDWTTPTDIGDTTNKSNEIPSTDVADQTNREH

Approximately 62-85 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.The protein migrates as 62-85 kDa under reducing condition (SDS-PAGE) due to glycosylation.

无菌粉末.

Lyophilized from a 0.2 μm solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.