TRP1 蛋白, Human (HEK293, His)

TRP1 (tyrosinase-related protein 1) is critical in melanin biosynthesis and catalyzes the oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) to indole-5,6-quinone-2- Carboxylic acids, especially in the presence of Cu(2+) ions. This activity is inhibited by Zn(2+). TRP1 Protein, Human (HEK293, His) is the recombinant human-derived TRP1 protein, expressed by HEK293 , with C-His labeled tag. The total length of TRP1 Protein, Human (HEK293, His) is 447 a.a., with molecular weight of ~60-75 kDa.

TRP1（酪氨酸酶相关蛋白 1）在黑色素生物合成中发挥着至关重要的作用，其参与 5,6-二羟基吲哚-2-羧酸 (DHICA) 氧化成吲哚-5,6-醌-2-羧酸就证明了这一点酸，特别是在存在结合的 Cu(2+) 离子的情况下。值得注意的是，这种酶活性在 Zn(2+) 存在下受到抑制。TRP1 参与调节黑色素合成的类型，从而影响色素沉着过程。此外，在较小程度上，TRP1 对酪氨酸表现出羟基化活性，有助于黑色素的产生。TRP1 的多方面功能强调了它在黑色素生成中的重要性，并强调了它在决定皮肤中生成的黑色素特征方面的潜在作用。

Measured by its ability to catalyze the formation of dopachrome from L-dopa. The specific activity is 73.524 U/mg, as measured under the described conditions.

Human

HEK293

C-10*His

P17643 (Q25-R471)

7306  [NCBI]

QFPRQCATVEALRSGMCCPDLSPVSGPGTDRCGSSSGRGRCEAVTADSRPHSPQYPHDGRDDREVWPLRFFNRTCHCNGNFSGHNCGTCRPGWRGAACDQRVLIVRRNLLDLSKEEKNHFVRALDMAKRTTHPLFVIATRRSEEILGPDGNTPQFENISIYNYFVWTHYYSVKKTFLGVGQESFGEVDFSHEGPAFLTWHRYHLLRLEKDMQEMLQEPSFSLPYWNFATGKNVCDICTDDLMGSRSNFDSTLISPNSVFSQWRVVCDSLEDYDTLGTLCNSTEDGPIRRNPAGNVARPMVQRLPEPQDVAQCLEVGLFDTPPFYSNSTNSFRNTVEGYSDPTGKYDPAVRSLHNLAHLFLNGTGGQTHLSPNDPIFVLLHTFTDAVFDEWLRRYNADISTFPLENAPIGHNRQYNMVPFWPPVTNTEMFVTAPDNLGYTYEIQWPSR

Approximately 60-75 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.