Trypsin 蛋白, Pig (P.pastoris, His)

Trypsin is a digestive endopeptidase that catalyzes the hydrolysis of peptide bonds on the carboxyl side of lysine or arginine residues. Trypsin is synthesized in the acinar cells of the pancreas. Trypsin is converted to the active enzyme in the gut. Trypsin Protein, Pig (P.pastoris, His) is the recombinant pig-derived Trypsin protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of Trypsin Protein, Pig (P.pastoris, His) is 223 a.a., with molecular weight of ~25.5 kDa.

胰蛋白酶是一种对赖氨酸或精氨酸残基羧基侧肽键水解进行催化的消化内肽酶。胰蛋白酶在胰腺腺泡细胞中合成，是一种无活性的前体，胰蛋白酶原。胰蛋白酶通过高度特异性的蛋白酶肠激酶在肠道中转化为活性酶。一旦被激活，胰蛋白酶会激活自身的酶原以及其他胰酶原。胰蛋白酶在食物消化以及通过 PAR 蛋白水解激活介导的细胞信号转导中起关键作用^[1][2]。

Pig

P. pastoris

N-6*His

P00761 (I9-N231)

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IVGGYTCAANSIPYQVSLNSGSHFCGGSLINSQWVVSAAHCYKSRIQVRLGEHNIDVLEGNEQFINAAKIITHPNFNGNTLDNDIMLIKLSSPATLNSRVATVSLPRSCAAAGTECLISGWGNTKSSGSSYPSLLQCLKAPVLSDSSCKSSYPGQITGNMICVGFLEGGKDSCQGDSGGPVVCNGQLQGIVSWGYGCAQKNKPGVYTKVCNYVNWIQQTIAAN

Approximately 25.5 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.