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  4. TSPAN1 蛋白, Human (HEK293, rFc)

TSPAN1 蛋白, Human (HEK293, rFc)

目录号: HY-P77261
产品使用指南

TSPAN1 蛋白是四跨膜蛋白 (TSPAN/TM4SF) 蛋白家族的一员，此家族重要特征是能够与彼此或各种其他跨膜受体聚集，成为富含 TSPAN 的微结构域 (TEM)。TSPAN1 在许多癌症类型中发挥癌蛋白的作用，尤其是消化道恶性肿瘤，如肝细胞癌 (HCC)、胰腺癌、胃癌、结直肠癌和食道癌。TSPAN1 是一种新型的巨自噬/自噬正调节因子，其特征是降低 LC3-II 和 SQSTM1/p62 表达，抑制 GFP-LC3 的斑点形成和自噬空泡。TSPAN1 蛋白, Human (HEK293, rFc) 是重组的 TSPAN1 蛋白，由 HEK293 表达，带有 N-rFc 标签。TSPAN1 蛋白, Human (HEK293, rFc) 全长 102 个氨基酸，分子量为 43-53 kDa。

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规格 是否有货
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100 μg   询价  

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描述

TSPAN1 Protein is a member of the tetraspanin (TSPAN/TM4SF) family of proteins whose important feature is their ability to aggregate with one another or various other transmembrane receptors, to become TSPAN-enriched microdomains (TEMs). TSPAN1 functions as an oncoprotein in many cancer types, especially digestive malignancies such as hepatocellular carcinoma (HCC), pancreatic, gastric, colorectal, and esophageal cancers. TSPAN1 is a novel positive regulator of macroautophagy/autophagy characterized by decreased LC3-II and SQSTM1/p62 expressions, inhibited puncta formation of GFP-LC3 and autophagic vacuoles[1][2][3]. TSPAN1 Protein, Human (HEK293, rFc) is the recombinant human-derived TSPAN1 protein, expressed by HEK293 , with N-rFc labeled tag. The total length of TSPAN1 Protein, Human (HEK293, rFc) is 102 a.a., with molecular weight of 43-53 kDa.
研究背景

TSPAN1 在胰腺癌中表达上调，并且 TSPAN1 缺失会降低体外和体内胰腺癌细胞的增殖。TSPAN1 通过两个保守的 LIR 基序直接与 LC3 结合，促进自噬成熟。TSPAN1 LIR 基序的突变导致诱导自噬和促进胰腺癌增殖的能力丧失[1]。
亲代和 CDDP 耐药 HNSCC 细胞中 TSPAN1 的缺失会减少细胞增殖、诱导细胞凋亡、减少自噬、对化疗药物敏感并抑制多种信号级联反应，其中磷酸化 SRC 抑制是主要的靶标[2]。
种属

Human
表达系统

HEK293
标签

N-rFc
蛋白编号

O60635 (Y110-N211)
基因 ID
蛋白结构
N-term
rFc
TSPAN1 (Y110-N211)
Accession # O60635
C-term
同用名
Tetraspanin-1; Tspan-1; Tetraspan NET-1; Tetraspanin TM4-C
分子量

43-53 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

TSPAN1 Protein, Human (HEK293, rFc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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