1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. TTN 蛋白, Human (His)

TTN 蛋白, Human (His)

目录号: HY-P71698
产品使用指南

TTN 蛋白对于脊椎动物横纹肌组装至关重要,建立对肌节力平衡至关重要的微丝连接。TTN 协调交联大小和延伸性,塑造肌节延伸性和肌肉功能。TTN 蛋白, Human (His) 是重组的 TTN 蛋白,由 E. coli 表达,带有 N-His 标签。TTN 蛋白, Human (His) 全长 207 个氨基酸,分子量约为 ~26.5 kDa。

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描述

The TTN protein is critical for vertebrate striated muscle assembly, establishing microfilament connections that are critical for sarcomeric force balance. TTN coordinates cross-link size and extensibility, shaping sarcomere extensibility and muscle function. TTN Protein, Human (His) is the recombinant human-derived TTN protein, expressed by E. coli , with N-His labeled tag. The total length of TTN Protein, Human (His) is 207 a.a., with molecular weight of ~26.5 kDa.

研究背景

TTN 是脊椎动物横纹肌组装和操作的关键参与者,通过在个体微丝水平建立连接而发挥着关键作用。这种贡献有助于维持肌节内力量的微妙平衡。由 TTN 精心安排的交联的大小和可伸展性成为塑造肌节可伸展性以及最终肌肉功能的关键决定因素。除了其以肌为中心的职责之外,TTN还将其影响扩展到非肌肉细胞,它似乎参与了有丝分裂过程中染色体浓缩和分离等重要过程。在这种情况下,TTN 的潜在作用包括在细胞周期的间期期间充当层网络和染色质或核肌动蛋白或两者之间的联系。这种多方面的参与强调了 TTN 在肌肉生理学和细胞动力学中的重要性。

种属

Human

表达系统

E. coli

标签

N-His

蛋白编号

Q8WZ42 (5398V-5604T)

基因 ID
蛋白结构
N-term
His
TTN (5398V-5604T)
Accession # Q8WZ42
C-term
同用名
MPRM; Cardiomyopathy dilated 1G CMD1G; CMH 9; CMH9; CMPD 4; CMPD4; Connectin; HMERF; LGMD2J; MU RMS 40.14; MYLK5; TMD; TTN
氨基酸序列

VFKCSVIGIPTPEVKWYKEYMCIEPDNIKYVISEEKGSHTLKIRNVCLSDSATYRCRAVNCVGEAICRGFLTMGDSEIFAVIAKKSKVTLSSLMEELVLKSNYTDSFFEFQVVEGPPRFIKGISDCYAPIGTAAYFQCLVRGSPRPTVYWYKDGKLVQGRRFTVEESGTGFHNLFITSLVKSDEGEYRCVATNKSGMAESFAALTLT

分子量

Approximately 26.5 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

TTN Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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TTN Protein, Human (His)
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