1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. TYR 蛋白, Human (HEK293, His)

TYR 蛋白, Human (HEK293, His)

目录号: HY-P79018
COA 产品使用指南

TYR 蛋白是一种含铜氧化酶,通过催化酪氨酸羟基化为多巴来启动黑色素的产生。这一重要步骤是黑色素和多酚化合物合成的限速。TYR 进一步能够将 DOPA 氧化为 DOPA-quinone,并可能介导 DHI 转化为 indole-5,6 quinone,在黑色素生物合成所涉及的复杂级联反应中发挥着至关重要的作用。TYR 蛋白, Human (HEK293, His) 是重组的 TYR 蛋白,由 HEK293 表达,带有 N-6*His 标签。

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描述

TYR protein, a copper-containing oxidase, initiates melanin production by catalyzing the hydroxylation of tyrosine to DOPA. This essential step is rate-limiting in the synthesis of melanins and polyphenolic compounds. TYR further enables the oxidation of DOPA to DOPA-quinone and potentially mediates the conversion of DHI to indole-5,6 quinone, playing a crucial role in the intricate cascade of reactions involved in melanin biosynthesis. TYR Protein, Human (HEK293, His) is the recombinant human-derived TYR protein, expressed by HEK293 , with N-6*His labeled tag.

研究背景

TYR蛋白是一种对色素形成至关重要的含铜氧化酶,在黑色素和各种多酚化合物的合成中发挥着关键作用。作为酪氨酸黑色素生成途径的引发剂,TYR 催化初始限速步骤,将酪氨酸羟基化为 DOPA(3,4-二羟基苯丙氨酸)。此外,它促进多巴氧化为多巴醌,并可能介导 DHI(5,6-二羟基吲哚)氧化为吲哚-5,6 醌,从而促进黑色素生物合成中涉及的复杂级联反应。

种属

Human

表达系统

HEK293

标签

N-6*His

蛋白编号

P14679-1 (H19-V377)

基因 ID
蛋白结构
N-term
6*His
TYR (H19-V377)
Accession # P14679-1
C-term
同用名
ATN; CMM8; LB24 AB; LB24-AB; OCA1; OCA1A; OCAIA; Oculocutaneous albinism IA; SHEP3; SK29 AB; SK29-AB; Tumor rejection antigen AB; TYR; Tyrosinase
氨基酸序列

HFPRACVSSKNLMEKECCPPWSGDRSPCGQLSGRGSCQNILLSNAPLGPQFPFTGVDDRESWPSVFYNRTCQCSGNFMGFNCGNCKFGFWGPNCTERRLLVRRNIFDLSAPEKDKFFAYLTLAKHTISSDYVIPIGTYGQMKNGSTPMFNDINIYDLFVWMHYYVSMDALLGGSEIWRDIDFAHEAPAFLPWHRLFLLRWEQEIQKLTGDENFTIPYWDWRDAEKCDICTDEYMGGQHPTNPNLLSPASFFSSWQIVCSRLEEYNSHQSLCNGTPEGPLRRNPGNHDKSRTPRLPSSADVEFCLSLTQYESGSMDKAANFSFRNTLEGFASPLTGIADASQSSMHNALHIYMNGTMSQV

分子量

Approximately 42 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

TYR Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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TYR Protein, Human (HEK293, His)
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