UAP1 蛋白, Human (P.pastoris, His)

UAP1 protein functions as an enzyme that catalyzes the conversion of UTP and GlcNAc-1-P into UDP-GlcNAc, as well as UTP and GalNAc-1-P into UDP-GalNAc. Notably, isoform AGX1 exhibits 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 displays 8 times more activity towards GlcNAc-1-P, highlighting distinct substrate preferences between the isoforms. UAP1 Protein, Human (P.pastoris, His) is the recombinant human-derived UAP1 protein, expressed by P. pastoris , with N-His labeled tag. The total length of UAP1 Protein, Human (P.pastoris, His) is 522 a.a., with molecular weight of ~60.8 kDa.

尿苷二磷酸-N-乙酰氨基葡萄糖 (UDP-GlcNAc) 和尿苷二磷酸-N-乙酰半乳糖胺 (UDP-GalNAc) 是参与糖基化过程的关键核苷酸糖，UAP1 蛋白在其合成中发挥核心作用。UAP1 与 GlcNAc-1-P 或 GalNAc-1-P 结合转化尿苷三磷酸 (UTP)，分别生成 UDP-GlcNAc 或 UDP-GalNAc。有趣的是，UAP1 的异构体 AGX1 对 GalNAc-1-P 的活性高出 2 至 3 倍，强调其对半乳糖胺的偏好，而异构体 AGX2 对 GlcNAc-1-P 的活性高出 8 倍，凸显其对 N-乙酰氨基葡萄糖的特异性。这些亚型的不同活性强调了 UAP1 在促进不同核苷酸糖的生成方面的多功能性，这对于细胞过程中的各种糖基化事件至关重要。

Human

P. pastoris

N-His

Q16222 (1M-522I)

6675  [NCBI]

MNINDLKLTLSKAGQEHLLRFWNELEEAQQVELYAELQAMNFEELNFFFQKAIEGFNQSSHQKNVDARMEPVPREVLGSATRDQDQLQAWESEGLFQISQNKVAVLLLAGGQGTRLGVAYPKGMYDVGLPSRKTLFQIQAERILKLQQVAEKYYGNKCIIPWYIMTSGRTMESTKEFFTKHKYFGLKKENVIFFQQGMLPAMSFDGKIILEEKNKVSMAPDGNGGLYRALAAQNIVEDMEQRGIWSIHVYCVDNILVKVADPRFIGFCIQKGADCGAKVVEKTNPTEPVGVVCRVDGVYQVVEYSEISLATAQKRSSDGRLLFNAGNIANHFFTVPFLRDVVNVYEPQLQHHVAQKKIPYVDTQGQLIKPDKPNGIKMEKFVFDIFQFAKKFVVYEVLREDEFSPLKNADSQNGKDNPTTARHALMSLHHCWVLNAGGHFIDENGSRLPAIPRSATNGKSETITADVNHNLKDANDVPIQCEISPLISYAGEGLESYVADKEFHAPLIIDENGVHELVKNGI

Approximately 60.8 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.