1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. UB2E2 蛋白, Human (Sf9, His, Strep)

UB2E2 蛋白, Human (Sf9, His, Strep)

目录号: HY-P701496
产品使用指南

UB2E2 蛋白是泛素-蛋白酶体系统的关键参与者,充当 E2 泛素结合酶,接受 E1 复合物中的泛素并催化其与蛋白质的共价附着。在体外,UB2E2 通过催化“Lys-11”、“Lys-48”和“Lys-63”连接的多泛素化而表现出多功能性,表明其参与多种细胞过程。UB2E2 蛋白, Human (Sf9, His, Strep) 是重组的 UB2E2 蛋白,由 Sf9 insect cells 表达,带有 N-Strep, N-8*His 标签。UB2E2 蛋白, Human (Sf9, His, Strep) 全长 200 个氨基酸。

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描述

The UB2E2 protein is a key ubiquitin-proteasome system participant, acting as an E2 ubiquitin-conjugating enzyme that accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to proteins. In vitro, UB2E2 exhibits multifunctionality by catalyzing polyubiquitination of “Lys-11,” “Lys-48,” and “Lys-63” linkages, suggesting its involvement in diverse cellular processes. UB2E2 Protein, Human (Sf9, His, Strep) is the recombinant human-derived UB2E2 protein, expressed by Sf9 insect cells , with N-Strep, N-8*His labeled tag. The total length of UB2E2 Protein, Human (Sf9, His, Strep) is 200 a.a., .

研究背景

UB2E2 是泛素-蛋白酶体系统中的关键参与者,充当 E2 泛素结合酶,从 E1 复合物中接受泛素并催化其与其他蛋白质的共价附着。在体外,UB2E2 通过催化“Lys-11”、“Lys-48”和“Lys-63”连接的多泛素化展示了其多功能性,表明其参与多种细胞过程。此外,UB2E2 显示出催化甲型流感病毒 NS1 蛋白 ISGylation 的独特能力,进一步扩展其功能库并突出其在抗病毒反应中的重要性。这种多方面的活性强调了 UB2E2 在细胞调节网络内的蛋白质修饰和降解途径中的关键作用。

种属

Human

表达系统

Sf9 insect cells

标签

N-Strep;N-8*His

蛋白编号

Q96LR5 (S2-T201)

基因 ID

7325

蛋白结构
N-term
8*His-Strep
UB2E2 (S2-T201)
Accession # Q96LR5
C-term
同用名
UBE2E2; Ubiquitin-conjugating enzyme E2 E2; E2 ubiquitin-conjugating enzyme E2; UbcH8; Ubiquitin carrier protein E2; Ubiquitin-protein ligase E2
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

UB2E2 Protein, Human (Sf9, His, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
UB2E2 Protein, Human (Sf9, His, Strep)
目录号:
HY-P701496
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