UbcH5b/UBE2D2 蛋白, Human

The UbcH5b/UBE2D2 protein is an important cellular mediator that accepts ubiquitin from the E1 complex and catalyzes multifunctional “Lys-48”-linked polyubiquitination on target proteins. In addition to its catalytic role, it also concentrates and selectively degrades short-lived proteins and contributes to E6/E6-AP-induced ubiquitination of p53/TP53. UbcH5b/UBE2D2 Protein, Human is the recombinant human-derived UbcH5b/UBE2D2 protein, expressed by E. coli , with tag free. The total length of UbcH5b/UBE2D2 Protein, Human is 147 a.a., with molecular weight of ~16 kDa.

UbcH5b/UBE2D2 蛋白是细胞过程中的关键介质，接受 E1 复合物中的泛素并催化其与各种蛋白质的共价附着。UbcH5b/UBE2D2 对“Lys-48”连接的多聚泛素化有明显的偏好，证明了其在修饰靶蛋白方面的多功能性。除了其催化作用之外，它还在短寿命和异常蛋白质的选择性降解中发挥着核心作用。UbcH5b/UBE2D2 在 E6/E6-AP 诱导的 p53/TP53 泛素化中发挥重要作用，并参与 PEX5 泛素化以及 STUB1 和 TRAF6 的自身泛素化。它的参与延伸到信号诱导的过程，包括 NFKBIA 的缀合和随后的降解、FBXW2 介导的 GCM1 泛素化和降解、p53/TP53 的 MDM2 依赖性降解，以及 RIGI 响应病毒感染而激活线粒体中的 MAVS 。此外，UbcH5b/UBE2D2 被证明对于 IRF3 的病毒激活至关重要。

Recombinant Human UbcH5b/UBE2D2 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins.

Human

E. coli

Tag Free

P62837-1 (M1-M147)

7322  [NCBI]

MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAM

Approximately 16 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.22 μm filtered solution of 20 mM HEPES, 50 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.