UBE1L2/UBA6 蛋白, Human (Sf9, His, Strep)

The ubiquitin E1 enzyme initiates ubiquitination by adenylating ubiquitin with ATP and forming a thioester bond with the E1 enzyme. It exhibits specificity for ubiquitin and excludes ubiquitin-like peptides. UBE1L2/UBA6 Protein, Human (Sf9, His, Strep) is the recombinant human-derived UBE1L2/UBA6 protein, expressed by Sf9 insect cells , with N-Strep, N-8*His labeled tag. The total length of UBE1L2/UBA6 Protein, Human (Sf9, His, Strep) is 1051 a.a., .

泛素 E1 酶通过两步机制激活泛素来启动泛素化过程。它首先用 ATP 将泛素的 C 端甘氨酸残基腺苷酸化，然后在该甘氨酸残基和 E1 酶中的半胱氨酸残基之间形成硫酯键，在此过程中释放出游离的 AMP。值得注意的是，它表现出对泛素的特异性，将泛素样肽排除在该激活过程之外。与 UBE1 相比，该酶对充电底物 E2 酶具有选择性，不包括细胞周期 E2，例如 CDC34。它在胚胎发育中的作用强调了它的不可或缺性，并且它对于 UBD/FAT10 的结合至关重要。该酶的亚型 2 可能对泛素系统产生重大影响，可能影响精子发生和男性生育能力。

Human

Sf9 insect cells

N-Strep;N-8*His

A0AVT1 (E2-D1052)

55236

EGSEPVAAHQGEEASCSSWGTGSTNKNLPIMSTASVEIDDALYSRQRYVLGDTAMQKMAKSHVFLSGMGGLGLEIAKNLVLAGIKAVTIHDTEKCQAWDLGTNFFLSEDDVVNKRNRAEAVLKHIAELNPYVHVTSSSVPFNETTDLSFLDKYQCVVLTEMKLPLQKKINDFCRSQCPPIKFISADVHGIWSRLFCDFGDEFEVLDTTGEEPKEIFISNITQANPGIVTCLENHPHKLETGQFLTFREINGMTGLNGSIQQITVISPFSFSIGDTTELEPYLHGGIAVQVKTPKTVFFESLERQLKHPKCLIVDFSNPEAPLEIHTAMLALDQFQEKYSRKPNVGCQQDSEELLKLATSISETLEEKPDVNADIVHWLSWTAQGFLSPLAAAVGGVASQEVLKAVTGKFSPLCQWLYLEAADIVESLGKPECEEFLPRGDRYDALRACIGDTLCQKLQNLNIFLVGCGAIGCEMLKNFALLGVGTSKEKGMITVTDPDLIEKSNLNRQFLFRPHHIQKPKSYTAADATLKINSQIKIDAHLNKVCPTTETIYNDEFYTKQDVIITALDNVEARRYVDSRCLANLRPLLDSGTMGTKGHTEVIVPHLTESYNSHRDPPEEEIPFCTLKSFPAAIEHTIQWARDKFESSFSHKPSLFNKFWQTYSSAEEVLQKIQSGHSLEGCFQVIKLLSRRPRNWSQCVELARLKFEKYFNHKALQLLHCFPLDIRLKDGSLFWQSPKRPPSPIKFDLNEPLHLSFLQNAAKLYATVYCIPFAEEDLSADALLNILSEVKIQEFKPSNKVVQTDETARKPDHVPISSEDERNAIFQLEKAILSNEATKSDLQMAVLSFEKDDDHNGHIDFITAASNLRAKMYSIEPADRFKTKRIAGKIIPAIATTTATVSGLVALEMIKVTGGYPFEAYKNCFLNLAIPIVVFTETTEVRKTKIRNGISFTIWDRWTVHGKEDFTLLDFINAVKEKYGIEPTMVVQGVKMLYVPVMPGHAKRLKLTMHKLVKPTTEKKYVDLTVSFAPDIDGDEDLPGPPVRYYFSHDTD

Approximately 121.0 KDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.22 μm filtered solution of 50 mM HEPES (pH7.5), 200 mM NaCl, 1 mM DTT, 20% glycerol.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.