UBE2B 蛋白, Human (HEK293, His)

The UBE2B protein is a key ubiquitination factor that acts as an E2 ubiquitin-conjugating enzyme, accepting ubiquitin from the E1 complex and catalyzing covalent attachment to proteins. UBE2B works in conjunction with the BRE1 (RNF20 and/or RNF40) E3 enzyme and plays a key role in transcriptional regulation by catalyzing the monoubiquitination of histone H2B at "Lys-120" (H2BK120ub1). UBE2B Protein, Human (HEK293, His) is the recombinant human-derived UBE2B protein, expressed by HEK293 , with C-6*His labeled tag. The total length of UBE2B Protein, Human (HEK293, His) is 152 a.a., with molecular weight of ~17.0 kDa.

UBE2B 是泛素化途径中的关键参与者，通过接受 E1 复合物中的泛素并催化其与其他蛋白质的共价连接，充当 E2 泛素结合酶。UBE2B 与 E3 酶 BRE1（RNF20 和/或 RNF40）合作，在转录调控中发挥着关键作用，催化组蛋白 H2B 在“Lys-120”（H2BK120ub1）处的单泛素化。这种修饰作为各种表观遗传过程的独特标签，包括转录激活、RNA 聚合酶 II 延伸和端粒沉默，并且对于 H3K4me 和 H3K79me 的形成至关重要。除了其组蛋白修饰功能外，UBE2B 还表现出多功能泛素链连接，在体外介导“Lys-11”、“Lys-48”和“Lys-63”连接的多泛素化。此外，UBE2B 在紫外线损伤 DNA 的复制后修复中发挥着至关重要的作用，并与 E3 连接酶 RAD18 协作形成 UBE2B-RAD18 泛素连接酶复合物，该复合物在“Lys-164”上单泛素化 DNA 相关 PCNA。UBE2B 的多种功能表明它可能参与神经突生长。

Human

HEK293

C-6*His

P63146 (M1-S152)

7320  [NCBI]

MSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWNDS

Approximately 17.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.