UBE2D1 蛋白, Human (GST)

The UBE2D1 protein is critical in cellular processes. It accepts ubiquitin from the E1 complex and preferentially catalyzes "Lys-48"-linked polyubiquitination in vitro, demonstrating multifunctionality. It mediates the selective degradation of short-lived and aberrant proteins and is involved in E6/E6-AP-induced ubiquitination of p53/TP53. UBE2D1 Protein, Human (GST) is the recombinant human-derived UBE2D1 protein, expressed by E. coli , with N-GST labeled tag. The total length of UBE2D1 Protein, Human (GST) is 147 a.a., with molecular weight of ~40.0 kDa.

UBE2D1 蛋白通过接受 E1 复合物中的泛素并促进其与多种蛋白质的共价连接，在细胞过程中发挥着关键作用（来源）。在体外，UBE2D1 表现出对“Lys-48”连接的多聚泛素化的催化偏好，展示了其在修饰靶蛋白方面的多功能性（来源）。UBE2D1 作为短寿命和异常蛋白选择性降解的关键介质，还参与 E6/E6-AP 诱导的 p53/TP53 泛素化。此外，它还促进 PEX5 的泛素化，并协调 STUB1、TRAF6 和 TRIM63/MURF1 的自动泛素化（来源）。UBE2D1 表现出在体外泛素化 STUB1 相关 HSP90AB1 的能力，强调了它参与复杂的细胞途径（来源）。值得注意的是，UBE2D1 对泛素的任何特定赖氨酸残基缺乏固有的特异性，突出了其在泛素修饰过程中的动态性质（来源）。它在 IRF3 病毒激活和 CYP3A4 多泛素化介导中的重要作用进一步强调了 UBE2D1 对细胞功能的多方面贡献（来源）。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-GST

P51668 (M1-M147)

7321  [NCBI]

MALKRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYPFKPPKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPDIAQIYKSDKEKYNRHAREWTQKYAM

Approximately 40.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 2 mM DTT, 10% Glycerin, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.