UBE2F 蛋白, Human (His, Solution)

The UBE2F protein accepts NEDD8 from the UBA3-NAE1 E1 complex and covalently links it to various target proteins in the ubiquitin-like NEDD8 conjugation pathway. The RBX2-UBE2F complex specifically interacts with the E3 ubiquitin ligase RBX2, but not RBX1, for the neddylation of specific targets, especially CUL5. UBE2F Protein, Human (His, Solution) is the recombinant human-derived UBE2F, expressed by E. coli , with N-6*His labeled tag. ,

UBE2F 蛋白在泛素样蛋白 NEDD8 缀合途径中发挥着至关重要的作用，从 UBA3-NAE1 E1 复合物中接受 NEDD8 并催化其与各种靶蛋白的共价附着。它与 E3 泛素连接酶 RBX2（而不是 RBX1）的独特相互作用意味着 RBX2-UBE2F 复合物专门用于特定靶蛋白（尤其是 CUL5）的 neddylation。这种特定的相互作用和 neddylation 活性凸显了 UBE2F 参与与关键底物的靶向泛素化相关的细胞过程的调节。

Measured in a cell proliferation assay using A427 cells. The ED₅₀ for this effect is 1.04 ng/mL, corresponding to a specific activity is 9.615×10⁵ units/mg.

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  Measured in a cell proliferation assay using A427 cells. The ED₅₀ for this effect is 1.04 ng/mL , corresponding to a specific activity is 9.615×10⁵ units/mg.

Human

E. coli

N-6*His

Q969M7-1 (M1-R185)

140739

MLTLASKLKRDDGLKGSRTAATASDSTRRVSVRDKLLVKEVAELEANLPCTCKVHFPDPNKLHCFQLTVTPDEGYYQGGKFQFETEVPDAYNMVPPKVKCLTKIWHPNITETGEICLSLLREHSIDGTGWAPTRTLKDVVWGLNSLFTDLLNFDDPLNIEAAEHHLRDKEDFRNKVDDYIKRYAR

Approximately 23 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4, 2 mM DTT, 10% Glycerol.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.