2. 重组蛋白
  3. Ubiquitin Related Proteins
  4. Ubiquitin Enzymes
  5. E2 Enzymes
  6. Ubiquitin-Conjugating Enzyme E2 Z
  7. UBE2Z 蛋白, Human (His)

UBE2Z 蛋白, Human (His)

目录号: HY-P71412
产品使用指南

UBE2Z 蛋白是泛素结合的催化剂，可促进泛素与靶蛋白的共价连接。它作为 UBA6 的特异性底物，参与细胞凋亡的调节。UBE2Z 蛋白, Human (His) 是重组的 UBE2Z 蛋白，由 E. coli 表达，带有 N-6*His 标签。UBE2Z 蛋白, Human (His) 全长 246 个氨基酸，分子量约为 ~32.0 kDa。

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100 μg   询价  

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描述

UBE2Z Protein, a catalyst in ubiquitin conjugation, facilitates the covalent attachment of ubiquitin to target proteins. It serves as a specific substrate for UBA6 and is implicated in the regulation of apoptosis. UBE2Z Protein, Human (His) is the recombinant human-derived UBE2Z protein, expressed by E. coli , with N-6*His labeled tag. The total length of UBE2Z Protein, Human (His) is 246 a.a., with molecular weight of ~32.0 kDa.
研究背景

UBE2Z 蛋白作为一种酶，催化泛素与其他蛋白质的共价附着，作为 UBA6 的底物，并且不被 UBE1 携带泛素。这意味着它参与了细胞凋亡调节的复杂过程，强调了它在泛素介导的靶蛋白修饰中的作用。
种属

Human
表达系统

E. coli
标签

N-6*His
蛋白编号

Q9H832-2 (M1-V246)
基因 ID
蛋白结构
N-term
6*His
UBE2Z (M1-V246)
Accession # Q9H832-2
C-term
同用名
Ubiquitin-Conjugating Enzyme E2 Z; Uba6-Specific E2 Conjugating Enzyme 1; Use1; Ubiquitin Carrier Protein Z; Ubiquitin-Protein Ligase Z; UBE2Z; HOYS7
氨基酸序列

MSIYKEPPPGMFVVPDTVDMTKIHALITGPFDTPYEGGFFLFVFRCPPDYPIHPPRVKLMTTGNNTVRFNPNFYRNGKVCLSILGTWTGPAWSPAQSISSVLISIQSLMTENPYHNEPGFEQERHPGDSKNYNECIRHETIRVAVCDMMEGKCPCPEPLRGVMEKSFLEYYDFYEVACKDRLHLQGQTMQDPFGEKRGHFDYQSLLMRLGLIRQKVLERLHNENAEMDSDSSSSGTETDLHGSLRV

分子量

Approximately 32.0 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

UBE2Z Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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