UCHL1 蛋白, Human (His)

The UCHL1 protein is a multifunctional deubiquitinase that regulates a variety of cellular processes. It maintains synaptic and cardiac function, modulates inflammatory responses, and influences osteoclastogenesis. UCHL1 Protein, Human (His) is the recombinant human-derived UCHL1 protein, expressed by E. coli , with C-6*His labeled tag. The total length of UCHL1 Protein, Human (His) is 223 a.a., with molecular weight of ~26.0 kDa.

UCHL1 是一种多方面的去泛素酶，协调多种细胞过程的调节，包括维持突触和心脏功能、调节炎症反应和破骨细胞生成。作为关键调节因子，UCHL1 干扰多种蛋白质的泛素化，例如 WWTR1/TAZ、EGFR、HIF1A 和 β 位点淀粉样蛋白前体蛋白裂解酶 1/BACE1。此外，它还能精确识别和水解泛素 C 端甘氨酸的肽键，确保维持稳定的单泛素库，这是泛素-蛋白酶体和自噬-溶酶体途径正常发挥作用的关键要求。UCHL1 的影响延伸至淀粉样蛋白前体蛋白/APP 加工，促进 BACE1 降解，从而导致 β 淀粉样蛋白产量减少。此外，UCHL1 通过微调 MHC I 分子向交叉呈递区室的运输来积极参与免疫反应，这对于生成 Ag-MHC I 复合物至关重要。通过“Lys-48”连接的去泛素化，它介导转录共激活因子 WWTR1/TAZ 的稳定，抑制破骨细胞生成。UCHL1 通过去泛素化和稳定 EGFR 以防止降解并激活下游介质，以及通过调节关键线粒体氧化蛋白来调节骨骼肌的氧化活性，进一步展示了其调节能力。此外，UCHL1 通过消除 VHL E3 连接酶介导的泛素化来增强缺氧诱导因子 1-α/HIF1A 的活性，从而抑制其降解。

Human

E. coli

C-6*His

P09936 (M1-A223)

7345  [NCBI]

MQLKPMEINPEMLNKVLSRLGVAGQWRFVDVLGLEEESLGSVPAPACALLLLFPLTAQHENFRKKQIEELKGQEVSPKVYFMKQTIGNSCGTIGLIHAVANNQDKLGFEDGSVLKQFLSETEKMSPEDRAKCFEKNEAIQAAHDAVAQEGQCRVDDKVNFHFILFNNVDGHLYELDGRMPFPVNHGASSEDTLLKDAAKVCREFTEREQGEVRFSAVALCKAA

Approximately 26.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 250 mM NaCl, 1 mM DTT, 10% Glycerol, pH 7.5 or 20 mM Tris-HCl, 250 mM NaCl, 10% Trehalose, 0.05% Tween 80, 1mM TCEP, pH 8.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.