UCHL3 蛋白, Mouse (His, solution)

UCHL3 Protein, a ubiquitin carboxyl-terminal hydrolase, is involved in the regulation of cellular processes and protein degradation. It is particularly expressed in the testes and plays a vital role in spermatogenesis. UCHL3 Protein's significance in male fertility and its potential as a therapeutic target in reproductive disorders make it a subject of interest in reproductive medicine. UCHL3 Protein, Mouse (His, solution) is the recombinant mouse-derived UCHL3 protein, expressed by E. coli , with N-His labeled tag. The total length of UCHL3 Protein, Mouse (His, solution) is 229 a.a., with molecular weight of ~26 kDa.

UCHL3 蛋白是一种去泛素化酶 (DUB)，通过处理泛素前体和泛素化蛋白，在控制细胞泛素水平方面发挥着至关重要的作用。作为一种硫醇蛋白酶，它特异性识别并水解泛素或 NEDD8 C 端甘氨酸的肽键。UCHL3 蛋白表现出对“Lys-48”连接的泛素链的偏好，并且对位置 P3'' 处的 Arg 和 Lys 具有 10 倍的偏好。其去泛素化活性包括顶端区室中 ENAC 的去泛素化，调节顶膜的回收。此外，UCHL3 蛋白还能间接增强 IGFIR、AKT 和 FOXO1 的磷酸化，从而促进胰岛素信号传导和胰岛素诱导的脂肪形成。它对于压力反应视网膜、骨骼肌和生殖细胞的维持也至关重要。此外，UCHL3 蛋白可能与工作记忆有关，并且可以水解 UBB(+1)，UBB(+1) 是泛素的一种突变形式，可抵抗蛋白酶体的降解。

Mouse

E. coli

N-His

Q9JKB1 (E2-A230)

50933

EGQRWLPLEANPEVTNQFLKQLGLHPNWQFVDVYGMEPELLSMVPRPVCAVLLLFPITEKYEVFRTEEEEKIKSQGQDVTSSVYFMKQTISNACGTIGLIHAIANNKDKMHFESGSTLKKFLEESVSMSPEERAKFLENYDAIRVTHETSAHEGQTEAPSIDEKVDLHFIALVHVDGHLYELDGRKPFPINHGKTSDETLLEDAIEVCKKFMERDPDELRFNAIALSAA

Approximately 26 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, 20% Glycerol, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.