USP14 蛋白, Human (His)

USP14 is a proteasome-associated deubiquitinase that regulates ubiquitin dynamics by releasing ubiquitin from proteins marked for degradation. As a reversible proteasome subunit, USP14 ensures ubiquitin replenishment. USP14 Protein, Human (His) is the recombinant human-derived USP14 protein, expressed by E. coli , with N-6*His labeled tag. The total length of USP14 Protein, Human (His) is 404 a.a., with molecular weight of 47-52 kDa.

USP14 是一种蛋白酶体相关的去泛素酶，在细胞过程中发挥着至关重要的作用，特别是在蛋白酶体泛素的动态调节中。作为蛋白酶体的可逆相关亚基，USP14 确保泛素化蛋白从目标降解中释放泛素，促进泛素在细胞环境中的再生。除了在蛋白酶体介导的蛋白质周转中发挥作用之外，USP14 在多种生理环境中也发挥着关键作用。它参与趋化因子受体 CXCR4 的降解，这是 CXCL12 诱导的细胞趋化性的关键事件。此外，USP14 在非应激条件下可作为内质网相关降解 (ERAD) 的生理抑制剂，与 ERN1 相互作用并调节未折叠内质网蛋白的降解。此外，USP14 有助于神经肌肉接头 (NMJ) 的突触发育和功能，并通过稳定病毒 DNA 传感器 CGAS 参与针对病毒的先天免疫防御，从而阻止其自噬降解。

Measured in a cell proliferation assay using A549 cells. The ED₅₀ for this effect is 13.76 ng/mL, corresponding to a specific activity is 7.27×10⁴ units/mg.

• 
  Measured in a cell proliferation assay using A549 cells. The ED₅₀ for this effect is 13.76 ng/mL, corresponding to a specific activity is 7.27×10⁴ units/mg.

Human

E. coli

N-6*His

P54578 (D91-Q494)

9097  [NCBI]

DMTEEQLASAMELPCGLTNLGNTCYMNATVQCIRSVPELKDALKRYAGALRASGEMASAQYITAALRDLFDSMDKTSSSIPPIILLQFLHMAFPQFAEKGEQGQYLQQDANECWIQMMRVLQQKLEAIEDDSVKETDSSSASAATPSKKKSLIDQFFGVEFETTMKCTESEEEEVTKGKENQLQLSCFINQEVKYLFTGLKLRLQEEITKQSPTLQRNALYIKSSKISRLPAYLTIQMVRFFYKEKESVNAKVLKDVKFPLMLDMYELCTPELQEKMVSFRSKFKDLEDKKVNQQPNTSDKKSSPQKEVKYEPFSFADDIGSNNCGYYDLQAVLTHQGRSSSSGHYVSWVKRKQDEWIKFDDDKVSIVTPEDILRLSGGGDWHIAYVLLYGPRRVEIMEEESEQ

47-52 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 100 mM NaCl, 20% Glycerol, pH 8.0 or 50 mM Tris-HCL, 300 mM NaCl, 200 mM arginine, pH 8.0, 20% Glycerol.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.