USP15 蛋白, Human (sf9)

USP15 is a hydrolase that complexly regulates cellular processes by deubiquitinating various target proteins in pathways such as TGF-β receptor, NF-κ-B, and RNF41/NRDP1-PRKN. In TGF-β signaling, it promotes R-SMAD deubiquitination or stabilizes TGFBR1, thereby enhancing pathway activity. USP15 Protein, Human (sf9) is the recombinant human-derived USP15 protein, expressed by Sf9 insect cells , with tag free. The total length of USP15 Protein, Human (sf9) is 980 a.a. .

USP15 是一种水解酶，通过从各种靶蛋白中去除缀合的泛素，在细胞调节中发挥着至关重要的作用，影响 TGF-β 受体信号传导、NF-κ-B 和 RNF41/NRDP1-PRKN 途径等途径。它对 TGF-β 受体信号通路的参与是多方面的；它可能促进单泛素化 R-SMAD 的去泛素化，减轻其抑制并激活 TGF-β 靶基因，或者可能介导 TGFBR1 的去泛素化和稳定化，增强 TGF-β 信号传导。USP15 表现出多种去泛素化能力，作用于单泛素化底物和不同类型的多泛素链。此外，它还通过抵消 Parkin 的作用，水解 MFN2 等靶蛋白上的多聚泛素链，充当线粒体自噬的抑制剂。USP15 还参与内体组织、NF-kappa-B 调节以及通过各种底物去泛素化来负向调节抗真菌免疫。此外，在微生物感染情况下，USP15 可通过泛素-蛋白酶体途径保护 APC 和人乳头瘤病毒 16 型蛋白 E6 免遭降解。

The fundamental role of USP15 is specific removal of ubiquitin from substrates. USP15 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535 nM emission light under the excitation condition of 485 nM. The signal of which can be quickly and reliably captured using a microplate reader.

Human

Sf9 insect cells

Tag Free

Q9Y4E8 (A2-N981)

9958

Approximately 112.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, 200 mM NaCl, 20% glycerol, 1 mM DTT, pH 7.5.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.