USP25 蛋白, Human

The USP25 protein acts as a deubiquitinating enzyme and plays a key role in the hydrolysis of the ubiquitin moiety conjugated to the substrate. This function enables processing of newly synthesized ubiquitin, recycling of ubiquitin molecules, and editing of polyubiquitin chains, thus preventing proteasomal degradation of substrates. USP25 Protein, Human is the recombinant human-derived USP25 protein, expressed by E. coli , with tag free. The total length of USP25 Protein, Human is 1054 a.a., .

泛素特异性蛋白酶 25 (USP25) 是一种去泛素化酶，能够水解与底物缀合的泛素部分，从而参与新合成泛素的加工、回收泛素分子和编辑多泛素链。USP25 通过裂解泛素链在防止底物蛋白酶体降解方面发挥着至关重要的作用。值得注意的是，它对“Lys-48”和“Lys-63”连接的四泛素链均表现出水解活性，展示了其针对不同泛素连接类型的多功能性。此外，USP25 的肌肉特异性亚型（称为 USP25m）被认为在肌肉分化和功能的调节中发挥作用，表明其在特定细胞环境中的潜在重要性。

The fundamental role of USP25 is specific removal of ubiquitin from substrates. USP25 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535 nM emission light under the excitation condition of 485 nM. The signal of which can be quickly and reliably captured using a microplate reader.

Human

E. coli

Tag Free

Q9UHP3 (T2-R1055)

29761

122.2 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH7.5, 200 mM NaCl, 20% glycerol.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.