USP28 蛋白, Human (Sf9, His)

The USP28 protein serves as a deubiquitinase and plays a key role in the DNA damage response checkpoint and the stability of the MYC proto-oncogene. It selectively deubiquitinates DNA damage pathway proteins such as CLSPN, thereby regulating apoptosis. USP28 Protein, Human (Sf9, His) is the recombinant human-derived USP28 protein, expressed by Sf9 insect cells , with N-8*His labeled tag. The total length of USP28 Protein, Human (Sf9, His) is 1076 a.a., .

USP28 蛋白作为本说明书的主题，作为脱泛素酶在 DNA 损伤反应检查点和 MYC 原癌基因的稳定性中发挥着关键作用。在 DNA 损伤诱导细胞凋亡的情况下，它特异性地去泛素化参与 DNA 损伤途径的蛋白质，例如 CLSPN，从而有助于调节这一关键的细胞过程。此外，USP28 通过去泛素化 CLSPN 参与 G2 DNA 损伤检查点，从而防止其被后期促进复合物/环体 (APC/C) 降解。值得注意的是，它在其靶标中表现出选择性，因为它不会使 PLK1 去泛素化。在核质中，USP28 通过特异性去泛素化 MYC，抵消 SCF(FBW7) 复合物的泛素化，在 MYC 稳定性中发挥关键作用。核质中与 FBXW7 亚型 1 的相互作用可防止 MYC 降解，而核仁中 FBXW7 亚型 1 的缺失则允许选择性 MYC 降解。此外，USP28 使 ZNF304 去泛素化，防止其被蛋白酶体降解，并有助于激活 KRAS 介导的启动子高甲基化和结直肠癌细胞子集中肿瘤抑制基因的转录沉默。这种多方面的作用凸显了 USP28 在关键细胞过程和致癌途径中复杂的调节功能。

Human

Sf9 insect cells

N-8*His

Q96RU2 (T2-K1077)

57646

124.5 kD

Greater than 80% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, 500 mM NaCl, 5% glycerol, 1 mM DTT, pH 8.0.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.