1. 重组蛋白
  2. Enzymes & Regulators Ubiquitin Related Proteins
  3. Hydrolases (EC 3) Deubiquitinase
  4. Ubiquitin-Specific Protease
  5. Ubiquitin-Specific Peptidase 29
  6. USP29 蛋白, Human (Sf9, FLAG)

USP29 蛋白, Human (Sf9, FLAG)

目录号: HY-P701449
产品使用指南

USP29 蛋白作为一种去泛素化酶,通过介导 CGAS 的“Lys-48”去泛素化,在先天抗病毒免疫中发挥着至关重要的作用。这种效应可以稳定 CGAS(病毒感染细胞反应的重要传感器),强调 USP29 在调节 CGAS 活性中的重要性。USP29 蛋白, Human (Sf9, FLAG) 是重组的 USP29 蛋白,由 Sf9 insect cells 表达,带有 N-Flag 标签。USP29 蛋白, Human (Sf9, FLAG) 全长 921 个氨基酸。

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描述

USP29 protein, as a deubiquitinating enzyme, plays a crucial role in innate antiviral immunity by mediating "Lys-48" deubiquitination of CGAS. This effect stabilizes CGAS, an important sensor of the cellular response to viral infection, emphasizing the importance of USP29 in regulating CGAS activity. USP29 Protein, Human (Sf9, FLAG) is the recombinant human-derived USP29 protein, expressed by Sf9 insect cells , with N-Flag labeled tag. The total length of USP29 Protein, Human (Sf9, FLAG) is 921 a.a., .

研究背景

作为本说明书的主题的USP29蛋白作为去泛素酶在先天抗病毒免疫中发挥着重要作用。它介导 CGAS 的“Lys-48”相关去泛素化,而 CGAS 是病毒感染细胞反应的关键参与者,从而导致 CGAS 的稳定。这种调节功能强调了 USP29 在调节 CGAS 活性中的重要性,CGAS 是先天免疫系统中的重要传感器。通过促进 CGAS 的去泛素化,USP29 有助于维持其稳定性,从而增强其识别和应对细胞环境中病毒威胁的有效性。

种属

Human

表达系统

Sf9 insect cells

标签

N-Flag

蛋白编号

Q9HBJ7 (I2-A922)

基因 ID

57663

蛋白结构
N-term
Flag
USP29 (I2-A922)
Accession # Q9HBJ7
C-term
同用名
USP29; Ubiquitin carboxyl-terminal hydrolase 29; Deubiquitinating enzyme 29; Ubiquitin thioesterase 29; Ubiquitin-specific-processing protease 29
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
USP29 Protein, Human (Sf9, FLAG)
目录号:
HY-P701449
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