USP46 蛋白, Human

USP46 protein, as a deubiquitinating enzyme, may participate in behavioral regulation by regulating the effects of GABA. Suggested to mediate deubiquitination of GAD1/GAD67, affecting processes related to GABAergic neurotransmission. USP46 Protein, Human is the recombinant human-derived USP46 protein, expressed by E. coli , with tag free. The total length of USP46 Protein, Human is 366 a.a., .

USP46 被确定为一种行为相关的去泛素化酶，可能通过调节 GABA 神经传递来影响行为。其作用被认为涉及介导 GAD1/GAD67 的去泛素化，这在 GABA 合成中至关重要。值得注意的是，USP46 独立地表现出最小的去泛素化活性，需要与 WDR48 相互作用才能发挥重要的酶功能。与 WDR48 的这种错综复杂的合作关系凸显了其酶活性的协作性质。值得注意的是，USP46 不参与单泛素化 FANCD2 的去泛素化，表明其细胞功能的特异性。

The fundamental role of USP46 is specific removal of ubiquitin from substrates. USP46 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535nM emission light under the excitation condition of 485nM.

Human

E. coli

Tag Free

P62068 (M1-E366)

64854

MTVRNIASICNMGTNASALEKDIGPEQFPINEHYFGLVNFGNTCYCNSVLQALYFCRPFRENVLAYKAQQKKKENLLTCLADLFHSIATQKKKVGVIPPKKFISRLRKENDLFDNYMQQDAHEFLNYLLNTIADILQEEKKQEKQNGKLKNGNMNEPAENNKPELTWVHEIFQGTLTNETRCLNCETVSSKDEDFLDLSVDVEQNTSITHCLRDFSNTETLCSEQKYYCETCCSKQEAQKRMRVKKLPMILALHLKRFKYMEQLHRYTKLSYRVVFPLELRLFNTSSDAVNLDRMYDLVAVVVHCGSGPNRGHYITIVKSHGFWLLFDDDIVEKIDAQAIEEFYGLTSDISKNSESGYILFYQSRE

Approximately 42.5 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.22 μm filtered solution of 50 mM HEPES (pH 7.5), 200 mM NaCl, 10% glycerol, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.