USP47 蛋白, Human (Sf9, FLAG)

The USP47 protein is a ubiquitin-specific protease that deubiquitinates monoubiquitinated DNA polymerase beta (POLB), stabilizes POLB, and regulates base excision repair (BER). In addition to DNA repair, USP47 is an important regulator of cell growth and genome integrity. USP47 Protein, Human (Sf9, FLAG) is the recombinant human-derived USP47 protein, expressed by Sf9 insect cells , with C-Flag labeled tag. The total length of USP47 Protein, Human (Sf9, FLAG) is 1374 a.a., .

USP47 蛋白作为泛素特异性蛋白酶，在单泛素化 DNA 聚合酶 β (POLB) 去泛素化中发挥特定作用，从而稳定 POLB 并有助于碱基切除修复 (BER) 的调节。除了在 DNA 修复中的作用之外，USP47 还成为细胞生长和基因组完整性的关键调节因子。此外，它可能通过在转录水平间接调节 CDC25A 表达来对细胞过程产生影响，强调其在细胞稳态和基因组稳定性中的多方面作用。

The fundamental role of USP47 is specific removal of ubiquitin from substrates. USP47 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535 nM emission ligat under the excitation condition of 485 nM. The signal of which can be quickly and reliably captured using a microplate reader.

Human

Sf9 insect cells

C-Flag

Q96K76 (V2-D1375)

55031

Approximately 158.3 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, 200 mM NaCl, 20% glycerol, pH 7.5, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.